Characterization of uropathogenic Escherichia coli strains obtained from urology outpatient clinic of Ege Medical Faculty in İzmir

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dc.contributor.authorGiray B.
dc.contributor.authorUçar F.B.
dc.contributor.authorAydemir S.S.
dc.date.accessioned2019-10-27T08:24:23Z
dc.date.available2019-10-27T08:24:23Z
dc.date.issued2012
dc.departmentEge Üniversitesien_US
dc.description.abstractAim: To identify the papG gene and its allelic variation in uropathogenic Escherichia coli (UPEC) strains isolated from patients with acute pyelonephritis and cystitis. Materials and methods: Seventy-five E. coli strains isolated from patients admitted to the University of Ege Medical Faculty urology outpatient clinic were isolated and identified phenotypically. All of these strains were examined for the papG gene and allelic distribution of this gene with the multiplex polymerase chain reaction technique. Results: papG genes were found in 24 of 75 E. coli strains. Of these 24 strains, 7 (29%) had papG class II only, 8 (33%) had class III only, and 9 (38%) had both class II and III. Phylogenetically, it was found that 31 belonged to group B2, 19 to group D, 20 to group A, and 5 to group Bl. Serotyping was performed and the positivity was found to be 39%. When the antibiotic resistance profiles of the 75 strains were evaluated, 41 (55%) of them were found to be resistant to ampicillin, 35 (47%) to ciprofloxacin, and 35 (47%) to trimethoprim/sulfamethoxazole. In addition, 23 strains (31%) produced extended-spectrum beta-lactamase. Conclusion: In this study, the rate of papG-positive strains was found to be low. However, there is no consensus on the molecular definition of UPEC. Although the presence of the papG gene indicates that the strains are UPEC, absence of the papG gene does not suggest that the strains are not UPEC. © TÜBİTAK.en_US
dc.identifier.doi10.3906/sag-1201-31
dc.identifier.endpage1337en_US
dc.identifier.issn1300-0144
dc.identifier.issn1300-0144en_US
dc.identifier.issueSUPPL.1en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1328en_US
dc.identifier.urihttps://doi.org/10.3906/sag-1201-31
dc.identifier.urihttps://hdl.handle.net/11454/26570
dc.identifier.volume42en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.relation.ispartofTurkish Journal of Medical Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectE. colien_US
dc.subjectPapGen_US
dc.subjectPhylogenetic groupingen_US
dc.subjectUPECen_US
dc.subjectUrinary tract infectionsen_US
dc.titleCharacterization of uropathogenic Escherichia coli strains obtained from urology outpatient clinic of Ege Medical Faculty in İzmiren_US
dc.typeArticleen_US

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