Characterization of Various Hypericum perforatum Clones by Hypericin and RAPD Analyses

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dc.contributor.authorTonk, Fatma Aykut
dc.contributor.authorGiachino, R. Refika Akcali
dc.contributor.authorSonmez, Cigdem
dc.contributor.authorYuce, Suer
dc.contributor.authorBayram, Emine
dc.contributor.authorTelci, Isa
dc.contributor.authorFuran, M. Alp
dc.date.accessioned2019-10-27T21:26:27Z
dc.date.available2019-10-27T21:26:27Z
dc.date.issued2011
dc.departmentEge Üniversitesien_US
dc.description.abstractHypericum perforatum L. is a pharmaceutically important medicinal plant with antidepressant, antiviral, antibacterial and anticancer effects of its metabolites. The hypericin contents and genetic structures of superior H. perforatum clones were identified by spectrophotometric analysis and random amplified polymorphic DNA (RAPD) markers, respectively. At the first harvest the hypericin content of the clones was higher than second harvest and higher in 2007 compared to 2008. For initial screening, 38 random primers were used and 26 of them detected a total of 378 scorable fragments. Effective multiplex ratio (EMR), average heterozygosity (H(av)), marker index (MI), resolving power (RP) and polymorphic information content (PIC) of the primers were calculated for RAPD marker system. Genetic similarity value among the clones varied from 0.40 to 0.93 and the average genetic similarity values was determined as 0.60. Clustering based on RAPD data showed a partial relationship on a regional basis in the clones. Cluster analyses of hypericin contents and RAPD markers grouped the clones in two major clusters and significant correlations were observed between them. In conclusion, combined analysis of RAPD markers and hypericin content is an optimal approach for further progress and breeding programs. (C) 2011 Friends Science Publishersen_US
dc.description.sponsorshipScientific and Technical Research Council of TurkeyTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [106 O 440]en_US
dc.description.sponsorshipWe thank The Scientific and Technical Research Council of Turkey for financial support (Project No: 106 O 440).en_US
dc.identifier.endpage37en_US
dc.identifier.issn1560-8530
dc.identifier.issn1560-8530en_US
dc.identifier.issue1en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage31en_US
dc.identifier.urihttps://hdl.handle.net/11454/44966
dc.identifier.volume13en_US
dc.identifier.wosWOS:000287722400005en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherFriends Science Publen_US
dc.relation.ispartofInternational Journal of Agriculture and Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectHypericum perforatumen_US
dc.subjectSt. John's worten_US
dc.subjectHypericinen_US
dc.subjectGenetic diversityen_US
dc.subjectRAPDen_US
dc.titleCharacterization of Various Hypericum perforatum Clones by Hypericin and RAPD Analysesen_US
dc.typeArticleen_US

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