Time course analysis of ABA and non-ionic osmotic stress-induced changes in water status, chlorophyll fluorescence and osmotic adjustment in Arabidopsis thaliana wild-type (Columbia) and ABA-deficient mutant (aba2)

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dc.contributor.authorOzfidan, Ceyda
dc.contributor.authorTurkan, Ismail
dc.contributor.authorSekmen, Askim Hediye
dc.contributor.authorSeckin, Burcu
dc.date.accessioned2019-10-27T21:53:19Z
dc.date.available2019-10-27T21:53:19Z
dc.date.issued2013
dc.departmentEge Üniversitesien_US
dc.description.abstractIn the present study, we investigated time course changes of water status including relative water content (RWC), leaf osmotic potential (Psi(Pi)), stomatal conductance (g,), proline (Pro), chlorophyll fluorescence (F-v/F-m) and total chlorophyll content in the Arabidopsis thaliana under PEG-induced drought stress after exogenous ABA treatment. To a better explanation for the role of ABA in the water status of A. thaliana to drought stress, wild-type (Columbia) and ABA-deficient mutant (aba2) of A. thaliana were used in the present study. Moreover, three weeks old Arabidopsis seedlings were applied exogenously with 50 p,M ABA and exposed to drought stress induced by 40% PEG8000 (-0.73 MPa) for 6 h, 12 h and 24 h (hours). Our findings indicate that RWC of wild-type and aba2 started to decrease in the first 12 h and 6 h of PEG-induced drought stress, respectively. However, exogenous treatment of 50 mu M ABA increased their RWC under drought stress. On the other hand, while We of both genotypes started to decrease in the first 6 h of drought stress, these declines in We were prevented by ABA treatment under stress throughout the experiment; it was more pronounced in aba2 at 24h. While the highest increase in gs was obtained in aba2 after 24 h stress, ABA-induced highest decrease in g5 was obtained in the same genotype during 12 h, as compared to PEG-treated group alone. On the other hand, Pro content increased in all treatment groups of ABA-deficient mutant aba2 at 12 h and 24 h. However, Pro content in ABA+ PEG treated aba2 plants was higher than in PEG- and ABA-treated plants alone at the end of the 24 h. Drought stress decreased Fv/Fn, and total chlorophyll contents of both genotypes while 50 1.1,M ABA alleviated these reductions during drought stress, as compared to PEG stressed plants. On the other hand, 50 LIM ABA treatment alone did not create any remarkable effect on F-v/F-m, and total chlorophyll contents. These findings indicate that exogenous ABA showed an alleviative effect against damage of drought stress on relative water content, osmotic potential, stomatal conductance, proline, chlorophyll fluorescence and total chlorophyll content of both genotypes during 24h of drought stress treatment. (C) 2010 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TBAG 107T671]; Ege University Science and Technology Center (EBILTEM)Ege University [2008-B1L/007]en_US
dc.description.sponsorshipFinancial support for this work was provided by The Scientific and Technological Research Council of Turkey (TUBITAK; Project number: TBAG 107T671) and the Ege University Science and Technology Center (EBILTEM; Project number, 2008-B1L/007). The authors express great gratitude to Prof. Dr. Laszlo SZABADOS (Biological Research Center of Hungarian Academy of Sciences, Szeged, Hungary), for providing ABA-deficient mutants (aba2) of A. thaliana.en_US
dc.identifier.doi10.1016/j.envexpbot.2010.09.008
dc.identifier.endpage51en_US
dc.identifier.issn0098-8472
dc.identifier.issn0098-8472en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage44en_US
dc.identifier.urihttps://doi.org/10.1016/j.envexpbot.2010.09.008
dc.identifier.urihttps://hdl.handle.net/11454/47865
dc.identifier.volume86en_US
dc.identifier.wosWOS:000313463600007en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherPergamon-Elsevier Science Ltden_US
dc.relation.ispartofEnvironmental and Experimental Botanyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAbscisic aciden_US
dc.subjectArabidopsis thaliana ABA-deficient mutant (aba2)en_US
dc.subjectChlorophyll fluorescenceen_US
dc.subjectDrought stressen_US
dc.subjectProlineen_US
dc.subjectWater statusen_US
dc.titleTime course analysis of ABA and non-ionic osmotic stress-induced changes in water status, chlorophyll fluorescence and osmotic adjustment in Arabidopsis thaliana wild-type (Columbia) and ABA-deficient mutant (aba2)en_US
dc.typeArticleen_US

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