Biocompatible polymeric coatings do not inherently reduce the cytotoxicity of iron oxide nanoparticles

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dc.contributor.authorSendemir Urkmez, Aylin
dc.contributor.authorBayir, Ece
dc.contributor.authorBilgi, Eyup
dc.contributor.authorOzen, Mehmet Ozgun
dc.date.accessioned2019-10-27T11:22:03Z
dc.date.available2019-10-27T11:22:03Z
dc.date.issued2017
dc.departmentEge Üniversitesien_US
dc.description.abstractNanotechnology in biomedical research is an emerging and promising tool for different purposes, like high-resolution medical imaging, diagnostics, and targeted drug delivery. Although some experimental efforts focused on determination of the cytotoxicity of nanoparticles (NPs) are present, there are many controversial results. In this study, chitosan (CS)- and poly(acrylic acid) (PAA)-coated iron oxide nanoparticles (IONPs) were used to investigate the possible cytotoxicity of coated IONPs on model mammalian cell line SaOs-2 by evaluating cellular viability and membrane integrity. Increasing concentrations of IONPs increased the cytotoxic effect on SaOs-2 cells with both CS-and PAA-coated IONPs. Cell viability on day 3 was as low as 40% and 48% at 1000 mu M concentration for PAA- and CS-coated IONPs, respectively, in 1% FBS-supplemented media. Cytotoxicity determined by the released lactate dehydrogenase (LDH) was as high as 163% with 1000 mu M concentration of CS-IONPs, while there was no significant change in LDH release with PAA-coated IONPs at any concentration. This study reveals that IONPs coated with a biocompatible polymer, which are usually assumed to be nontoxic, show cytotoxicity with increasing concentration and incubation time. The cytotoxicity of nanoparticles intended for biomedical purposes must be evaluated using more than one approach.en_US
dc.identifier.doi10.3906/biy-1608-61
dc.identifier.endpage332en_US
dc.identifier.issn1300-0152
dc.identifier.issn1303-6092
dc.identifier.issn1300-0152en_US
dc.identifier.issn1303-6092en_US
dc.identifier.issue2en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage322en_US
dc.identifier.urihttps://doi.org/10.3906/biy-1608-61
dc.identifier.urihttps://hdl.handle.net/11454/32990
dc.identifier.volume41en_US
dc.identifier.wosWOS:000400179800008en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherTubitak Scientific & Technical Research Council Turkeyen_US
dc.relation.ispartofTurkish Journal of Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectIron oxide nanoparticlesen_US
dc.subjectpoly(acrylic acid)en_US
dc.subjectchitosanen_US
dc.subjecthuman osteoblast-like cell lineen_US
dc.subjectcytotoxicityen_US
dc.titleBiocompatible polymeric coatings do not inherently reduce the cytotoxicity of iron oxide nanoparticlesen_US
dc.typeArticleen_US

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