Radiolabeling of EGCG with I-131 and biodistribution in rats

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dc.contributor.authorToksoz, Feriha
dc.contributor.authorDemir, Ilknur
dc.contributor.authorBayrak, Elif
dc.contributor.authorKocagozoglu, Gamze
dc.contributor.authorOnursal, Mehmet
dc.contributor.authorKarademir, Gulgun
dc.contributor.authorLambrecht, Fatma Yurt
dc.date.accessioned2019-10-27T21:44:50Z
dc.date.available2019-10-27T21:44:50Z
dc.date.issued2012
dc.departmentEge Üniversitesien_US
dc.description.abstractEpigallocatechin gallate (EGCG), is the most abundant and widely studied catechin in green tea (Camellia sinensis Theaceae). The inhibitory effects of EGCG and green tea extract on carcinogenesis in various organs in rodents have now been demonstrated over the past decade. The aim of study was to label EGCG with I-131, to determinate its structure and to evaluate its biodistribution in Wistar rats. Radiolabeling was carried out by direct electrophilic iodination method (iodogen) and yield was determined by radio thin layer chromatography (RTLC). Radiolabelling yield is determined as 89 +/- A 1.0%. Besides, determination of structure of iodinated molecule, serum stability, and partition coefficient experiments was performed. The structure analysis of synthesized cold I-127-EGCG complex was assessed with LC-MS-MS and H-1-NMR. H-1-NMR and LC-MS-MS results of iodinated EGCG (I-127-EGCG) show that oxidize iodine reacts electrophilic with aromatic ring. Serum stability results showed that in vitro stability of I-131-EGCG was quite high. It is observed that labeling percentage decreased 83 +/- A 2% at 24th, Partition coefficient results show that the partition coefficient of EGCG was calculated as theoretical partition coefficient = 2.04 +/- A 0.42 and the experimental partition coefficient of I-131-EGCG was found as 1.46 +/- A 0.2. The biodistribution data shown that the maximum uptake of the radioiodinated EGCG was seen in lung and pancreas at 30 min. The blocking assay results indicated that the uptake of I-131-EGCG in lung was not significantly change (0.25, 0.23, and 0.22%ID/g at 30, 60, and 150 min, respectively). Biodistribution data showed no significant uptake in a specific organ of the rat. Hence radiolabeled EGCG is seen in some organs (lung, liver, pancreas, kidney, etc.).en_US
dc.identifier.doi10.1007/s00044-010-9535-7
dc.identifier.endpage228en_US
dc.identifier.issn1054-2523
dc.identifier.issn1054-2523en_US
dc.identifier.issue2en_US
dc.identifier.startpage224en_US
dc.identifier.urihttps://doi.org/10.1007/s00044-010-9535-7
dc.identifier.urihttps://hdl.handle.net/11454/47302
dc.identifier.volume21en_US
dc.identifier.wosWOS:000298882100011en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherBirkhauser Boston Incen_US
dc.relation.ispartofMedicinal Chemistry Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectEpigallocatechin gallateen_US
dc.subjectI-131en_US
dc.subjectIodogenen_US
dc.subjectEGCGen_US
dc.subjectPolyphenolen_US
dc.titleRadiolabeling of EGCG with I-131 and biodistribution in ratsen_US
dc.typeArticleen_US

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