A bioactivity based comparison of Echinacea purpurea extracts obtained by various processes

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dc.contributor.authorYildiz, Ece
dc.contributor.authorKarabulut, Dilan
dc.contributor.authorYesil-Celiktas, Ozlem
dc.date.accessioned2019-10-27T22:15:14Z
dc.date.available2019-10-27T22:15:14Z
dc.date.issued2014
dc.departmentEge Üniversitesien_US
dc.description.abstractEchinacea species is provided as dietary supplements for various infectious and immune related disorders and has a potential role in cancer prevention. The aim of this study was to optimize the extraction of total flavonoids using different extraction methods and investigate the cytotoxic effects on various cancer cell lines (CaCo-2, MCF-7, A549, U87MG, and HeLa) and VERO (African green monkey) as a non-cancerous cell line. Box-Behnken statistical design was used to evaluate the effect of pressure (100-200 bar), temperature (40-80 degrees C) and ethanol as co-solvent (6-20 wt%) at a flow rate of 15 g/min for 60 min in supercritical CO2 extraction and the effect of temperature (60-100 degrees C), time (5-15 min) and power (300-900W) in microwave-assisted extraction. Optimum extraction conditions were elicited as 300 bar, 80 degrees C and 13% co-solvent yielding 0.472 mg rutin equivalent total flavonoids/g extract in SC-CO2 extraction, whereas 60 degrees C, 10 min and 300W yielded the highest (0.202 mg rutin equivalent) total flavonoids in microwave-assisted extraction. Additional trials with subcritical water (0.022 mg/g) and Soxhlet extraction with methanol (0.238 mg/g) yielded lower flavonoid contents. The exposures upto 50 mu g/ml of extracts revealed no significant inhibition on the proliferation of both tested cancer cells and healthy VERO cells. (C) 2014 Elsevier B.V. All rights reserved.en_US
dc.identifier.doi10.1016/j.supflu.2014.02.005
dc.identifier.endpage15en_US
dc.identifier.issn0896-8446
dc.identifier.issn1872-8162
dc.identifier.issn0896-8446en_US
dc.identifier.issn1872-8162en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage8en_US
dc.identifier.urihttps://doi.org/10.1016/j.supflu.2014.02.005
dc.identifier.urihttps://hdl.handle.net/11454/50139
dc.identifier.volume89en_US
dc.identifier.wosWOS:000336351300002en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofJournal of Supercritical Fluidsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectEchinacea purpureaen_US
dc.subjectSupercritical CO2 extractionen_US
dc.subjectSubcritical water extractionen_US
dc.subjectMicrowave-assisted extractionen_US
dc.subjectFlavonoidsen_US
dc.subjectCytotoxic activityen_US
dc.titleA bioactivity based comparison of Echinacea purpurea extracts obtained by various processesen_US
dc.typeArticleen_US

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