Electrochemical detection of interaction between daunorubicin and DNA by hybrid nanoflowers modified graphite electrodes

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dc.contributor.authorFindik, M.
dc.contributor.authorBingol, H.
dc.contributor.authorErdem, A.
dc.date.accessioned2020-12-01T11:52:55Z
dc.date.available2020-12-01T11:52:55Z
dc.date.issued2020
dc.departmentEge Üniversitesien_US
dc.description.abstractIn this study, two types of hybrid nanoflowers (NFs) modified disposable electrodes were developed to investigate the electrochemical behavior of anticancer drug-DNA interaction. Biocompatible hybrid NFs were synthesized by amino acids (L-glutamic acid and L-cysteine) via a simple, rapid and cost-effective methods. Structural characterizations of the synthesized hybrid NFs were performed with Attenuated Total Reflection-Fourier Transform Infrared (ATR-FTIR), Raman spectroscopy and X-ray photoelectron spectroscopy (XPS), while morphological characterizations were performed using scanning electron microscopy (SEM) and energy diffuser X-ray (EDX) spectroscopy techniques. As the sensing platform in electrochemical studies, pencil graphite electrode (PGE) has been used because of its advantages such as low cost, easy-to-handle, portable and disposable. After PGEs modified with NFs were characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) techniques, differential pulse voltammetry (DPV) technique was used in the determination of calf thymus double stranded DNA (ctdsDNA) and also daunorubicin (DNR) as well as DNA-DNR interaction. The detection limits as a result of ctdsDNA determination were found to be 0.93 ?g/mL by using L-cysteine NFs modified PGE (c-NFs-PGE) and 2.37 ?g/mL by using L-glutamic acid modified PGE (ga-NFs-PGE). The detection limits obtained for the determination of DNR were found as 2.93 ?M and 2.06 ?M with c-NFs-PGE and ga-NFs-PGE, respectively. The optimum interaction time between DNR and ctdsDNA was determined by c-NFs-PGE, which showed higher sensitivity performance against oxidation of guanine signals. © 2020en_US
dc.description.sponsorshipTürkiye Bilimsel ve Teknolojik Araştirma Kurumu, TÜBITAKen_US
dc.description.sponsorshipM.F. acknowledges the postdoctoral scholarship under 2218- National Postdoctoral Research Fellowship Programme granted by The Scientific and Technological Research Council of Turkey (TÜBİTAK) . A.E. would like to express her gratitude to the Turkish Academy of Sciences (TÜBA) as a Principal member for its partial support.en_US
dc.identifier.doi10.1016/j.snb.2020.129120
dc.identifier.issn0925-4005
dc.identifier.issn0925-4005en_US
dc.identifier.scopus2-s2.0-85095763180en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.snb.2020.129120
dc.identifier.urihttps://hdl.handle.net/11454/61687
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevier B.V.en_US
dc.relation.ispartofSensors and Actuators, B: Chemicalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAnticancer drug-DNA interactionen_US
dc.subjectDaunorubicinen_US
dc.subjectElectrochemical sensorsen_US
dc.subjectHybrid nanoflowersen_US
dc.subjectPencil graphite electrodeen_US
dc.titleElectrochemical detection of interaction between daunorubicin and DNA by hybrid nanoflowers modified graphite electrodesen_US
dc.typeArticleen_US

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