Individual and combinative effect of NaCl and ?-radiation on NADPH-generating enzymes activity in corn (Zea mays L.) sprouts

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dc.authorscopusid58699728000
dc.authorscopusid41961222100
dc.authorscopusid55336212000
dc.authorscopusid56829076800
dc.authorscopusid57193308858
dc.contributor.authorAliyeva, N.
dc.contributor.authorNasibova, A.
dc.contributor.authorMammadov, Z.
dc.contributor.authorEftekhari, A.
dc.contributor.authorKhalilov, R.
dc.date.accessioned2024-08-25T18:51:23Z
dc.date.available2024-08-25T18:51:23Z
dc.date.issued2023
dc.departmentEge Üniversitesien_US
dc.description.abstractBeing a universal reducing agent nicotinamide adenine dinucleotide phosphate hydrogen (NADPH) plays an important role in the cellular metabolism and the implementation of anti-stress reactions in plants. There are only a few enzymes that ensure the NADPH pool formation in cells. Among them, the most important are glucose-6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49), malate dehydrogenase decarboxylating (DMDH, malic enzyme, EC 1.1.1.40) and NADP-isocitrate dehydrogenase (NADP-IDH, EC 1.1.1.42). The presented investigation is devoted to studying the influence of the individual and combinative effects of NaCl and ?-radiation as abiotic stress factors on biometric indicators and activity of these NADPH-generating enzymes, on organic content, and the formation of paramagnetic centers as defense reaction in corn (Zagatala-68 genotype) sprouts. It was found that 100 mM NaCl had an inhibitory effect on the development of sprouts. Relatively lower doses (50 Gy and 100 Gy) of ?-radiation had a positive, but its higher doses (150 Gy and 200 Gy) had a negative effect on this process. 500 Gy was a lethal dose (LD) for the corn sprouts. Combinative stress in all cases considerably delayed the development of sprouts. G6PDH showed the highest activity in the first, whereas, NADP-IDH showed the same activity in the last days of the experiment. All three enzymes, especially the G6PDH, have been activated in both root and stem tissues under the influence of stress factors (either radiation or salt). Combinative stress (?-radiation + salt) also led to an induction of these activities which was necessary to neutralize the negative consequences of stress factors. Stress factors in all cases also had a negative effect on the content of organic matter in seedlings. Ionizing gamma radiation, which resulted in the formation of new paramagnetic centers as an anti-stress defense reaction in many cases was observed in wheat seedlings, but not in corn sprouts, which clearly shows that there are some differences in the protective mechanisms of these C3- and C4-types of plants to ?-radiation. © 2023en_US
dc.identifier.doi10.1016/j.heliyon.2023.e22126
dc.identifier.issn2405-8440
dc.identifier.issue11en_US
dc.identifier.scopus2-s2.0-85177046726en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.heliyon.2023.e22126
dc.identifier.urihttps://hdl.handle.net/11454/102566
dc.identifier.volume9en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevier Ltden_US
dc.relation.ispartofHeliyonen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240825_Gen_US
dc.subjectCorn sproutsen_US
dc.subjectNADPH-Generating enzymesen_US
dc.subjectSalt stressen_US
dc.subject?-radiationen_US
dc.titleIndividual and combinative effect of NaCl and ?-radiation on NADPH-generating enzymes activity in corn (Zea mays L.) sproutsen_US
dc.typeArticleen_US

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