A novel immobilization matrix for the biosensing of phenol: Self assembled monolayers of calixarenes [Phenolün biyosensörle belirlenmesi için yeni bir immobilizasyon matriksi: Kaliksarenlerin kendiliğinden oluşan tek tabakaları]

Basit Öğe Kaydı
dc.contributor.authorTasci F.
dc.contributor.authorSayin S.
dc.contributor.authorSeleci D.A.
dc.contributor.authorDemir B.
dc.contributor.authorAzak H.
dc.contributor.authorYildiz H.B.
dc.contributor.authorDemirkol D.O.
dc.contributor.authorTimur S.
dc.date.accessioned2019-10-27T08:04:05Z
dc.date.available2019-10-27T08:04:05Z
dc.date.issued2017
dc.departmentEge Üniversitesien_US
dc.description.abstractAim: The development of calixarene based phenol biosensor. Methods: This study describes the application of a calixarene derivative, 5,17-diamino-25,27-bis(3-thiol-1-oxypropane)-26,28-dihydroxycalix[4]arene (HS-Calix-NH2) which has both amino and thiol functionalities, in the practical surface modifications for biomolecule binding. The structure of HS-Calix-NH2 allows easy interaction with Au surface and one-step biomolecule immobilization. Self-assembled monolayers (SAMs) of p-aminofunctionalized mercaptoalkylcalixarene (HS-Calix-NH2) were formed onto the Au electrode. Then, Laccase (Lac) enzyme was immobilized onto the modified surface by crosslinking with glutaraldehyde (GA). Resulted electrode (HS-Calix-NH2/Lac) was used for the electrochemical analysis of phenolic compounds at - 50 mV. Results: The linearity was observed in the range of 0.1-100 µM and 1.0-100 µM for catechol and phenol, respectively. The potential use of the biosensor was investigated for phenol analysis in artificial samples which simulate the industrial waste water, which is highly acidic and composed of concentrated salt, without needing any sample pre-treatment step. Conclusion: The prepared Lac biosensor has a potential for rapid, selective and easy detection of phenolic contaminations in samples. © 2017 Turkish Biochemistry Society. All rights reserved.en_US
dc.identifier.doi10.1515/tjb-2016-0178
dc.identifier.endpage236en_US
dc.identifier.issn0250-4685
dc.identifier.issn0250-4685en_US
dc.identifier.issue2en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage229en_US
dc.identifier.urihttps://doi.org/10.1515/tjb-2016-0178
dc.identifier.urihttps://hdl.handle.net/11454/25462
dc.identifier.volume42en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherTurkish Biochemistry Societyen_US
dc.relation.ispartofTurkish Journal of Biochemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAmperometric biosensoren_US
dc.subjectFunctionalized calixareneen_US
dc.subjectImmobilization of enzymesen_US
dc.subjectLaccaseen_US
dc.subjectPhenol detectionen_US
dc.titleA novel immobilization matrix for the biosensing of phenol: Self assembled monolayers of calixarenes [Phenolün biyosensörle belirlenmesi için yeni bir immobilizasyon matriksi: Kaliksarenlerin kendiliğinden oluşan tek tabakaları]en_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

Scopus İndeksli Yayınlar Koleksiyonu