Trace determination of DNA in an amaryllidaceae plant, Narcissus tazetta by square-wave stripping voltammetry based on guanine signal

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dc.contributor.authorYardim Y.
dc.contributor.authorŞentürk Z.
dc.contributor.authorÖzsöz M.
dc.contributor.authorÖzdemir K.
dc.contributor.authorAtalan E.
dc.date.accessioned2019-10-26T23:58:58Z
dc.date.available2019-10-26T23:58:58Z
dc.date.issued2007
dc.departmentEge Üniversitesien_US
dc.description.abstractThis study is, to our knowledge, the first application of electrochemical DNA biosensor for the quantification of DNA in plant extracts. The DNA biosensor was assembled by immobilizing the double-stranded fish sperm DNA on the surface of a singleuse pencil graphite electrode. Square-wave voltammetric method with the baseline correction was carried out to evaluate the oxidation signal of the guanine base. The experimental variables such as solution pH, buffer concentration, immobilization time, stirring speed, and square-wave parameters were optimized. The extremely low detection limit (0.36 ng/mL) was coupled to a good surface-to-surface reproducibility (a relative standard deviation of 8.4% for 7 repetitive measurements of 40 ng/mL). The renewable and low-cost DNA biosensor developed in this study using pencil graphite electrode was applied to the determination of DNA isolated from an Amaryllidaceae plant, Narcissus tazetta.en_US
dc.identifier.endpage165en_US
dc.identifier.issn1300-4182
dc.identifier.issn1300-4182en_US
dc.identifier.issue4en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage159en_US
dc.identifier.urihttps://hdl.handle.net/11454/21316
dc.identifier.volume32en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.relation.ispartofFabad Journal of Pharmaceutical Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectElectrochemical DNA biosensoren_US
dc.subjectNarcissus tazette subsp. tazettaen_US
dc.subjectPencil graphite electrodeen_US
dc.subjectPlant samplesen_US
dc.subjectSquare-wave stripping voltammetryen_US
dc.titleTrace determination of DNA in an amaryllidaceae plant, Narcissus tazetta by square-wave stripping voltammetry based on guanine signalen_US
dc.typeArticleen_US

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