A new bienzymatic biosensor based on butyrylcholine esterase-sulfhydryl oxidase enzymes

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dc.contributor.authorTeksoy, Sadik
dc.contributor.authorOdaci, Dilek
dc.contributor.authorTimur, Suna
dc.date.accessioned2019-10-27T19:37:00Z
dc.date.available2019-10-27T19:37:00Z
dc.date.issued2007
dc.departmentEge Üniversitesien_US
dc.description.abstractOver the last decades, cholinesterase (ChE) biosensors have emerged as a sensitive and rapid technique for toxicity analysis in environmental monitoring, food, and quality control. These systems have the potential to complement or replace the classical analytical methods by simplifying or eliminating sample preparation protocols and making field testing easier and faster with significant decrease in costs per analysis. In this study, a new bienzymatic biosensor based on butyrylcholinesterase (BuChE) and sulfhydryl oxidase (SOX) enzymes was developed. This system makes use of the biocatalyzed hydrolysis of butyrylthiocholine to butyric acid and thiocholine that acts as a SOX substrate. Measurements were performed by following of the consumed oxygen level related to butyrylthiocholine concentration in the enzymatic reactions. Bienzymatic system was characterized and applied for detection of donepezil which is a reversible inhibitor of cholinesterase and belongs to a new class of cholinesterase inhibitors having an N-benzylpiperidine and an indanone moiety, by following of the decrease in biosensor response as a result of competitive inhibition.en_US
dc.identifier.doi10.1080/00032710701603819
dc.identifier.endpage2850en_US
dc.identifier.issn0003-2719
dc.identifier.issn0003-2719en_US
dc.identifier.issue15en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage2840en_US
dc.identifier.urihttps://doi.org/10.1080/00032710701603819
dc.identifier.urihttps://hdl.handle.net/11454/39922
dc.identifier.volume40en_US
dc.identifier.wosWOS:000250481400003en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Incen_US
dc.relation.ispartofAnalytical Lettersen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectbutyrylcholinesterase (BuChE)en_US
dc.subjectsulfhydryl oxidase (SOX)en_US
dc.subjectbiosensoren_US
dc.titleA new bienzymatic biosensor based on butyrylcholine esterase-sulfhydryl oxidase enzymesen_US
dc.typeArticleen_US

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