Adsorption of lysozyme from aqueous solutions by a novel bentonite-tyrptophane (Bent-Trp) microcomposite affinity sorbent

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dc.contributor.authorKalburcu, Tulden
dc.contributor.authorTabak, Ahmet
dc.contributor.authorOzturk, Nevra
dc.contributor.authorTuzmen, Nalan
dc.contributor.authorAkgol, Sinan
dc.contributor.authorCaglar, Bulent
dc.contributor.authorDenizli, Adil
dc.date.accessioned2019-10-27T22:27:36Z
dc.date.available2019-10-27T22:27:36Z
dc.date.issued2015
dc.departmentEge Üniversitesien_US
dc.description.abstractThis study deals with developing bentonite-tryptophan (Bent-Trp) microcomposite affinity sorbent (38-105 gm) for lysozyme adsorption from aqueous solutions. The structural analysis results of Bent-Trp microcomposites obtained from X-ray diffraction (XRD), Fourier Transform Infrared (FTIR), thermal (TG-DTG/DTA) and elemental analysis techniques clearly pointed out that the pseudo-biospecific affinity ligand L-Tryptophan was penetrated into interlayer space of the bentonite by diplacing water molecules and an elemental analysis of immobilized L-Tryptophan for nitrogen was 541.3 mu mol g(-1) bentonite. The effects of initial concentration, pH, and temperature on the adsorption efficiency of this sorbent were also studied in a batch system. The maximum amount of lysozyme adsorption from aqueous solution was determined as 365.16 mg/g at pH 10.0 and 1.25 mg/mL initial concentration of lysozyme, while the non-specific adsorption of lysozyme was very low (4.21 mg g(-1) sorbent). We concluded that Bent-Trp microcomposite affinity sorbents could be repeatedly applied for lysozyme adsorption without significant losses in the adsorption capacity. (C) 2014 The Authors. Published by Elsevier B.V.en_US
dc.description.sponsorshipRecep Tayyip Erdogan UniversityRecep Tayyip Erdogan Universityen_US
dc.description.sponsorshipThe authors are grateful to the Recep Tayyip Erdogan University and its central laboratory for the financial and technical supports.en_US
dc.identifier.doi10.1016/j.molstruc.2014.11.055
dc.identifier.endpage162en_US
dc.identifier.issn0022-2860
dc.identifier.issn1872-8014
dc.identifier.issn0022-2860en_US
dc.identifier.issn1872-8014en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage156en_US
dc.identifier.urihttps://doi.org/10.1016/j.molstruc.2014.11.055
dc.identifier.urihttps://hdl.handle.net/11454/50718
dc.identifier.volume1083en_US
dc.identifier.wosWOS:000348879500021en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofJournal of Molecular Structureen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBentonite-tyrptophane microcompositeen_US
dc.subjectLysozymeen_US
dc.subjectAffinity chromatographyen_US
dc.subjectTG-DTG/DTAen_US
dc.subjectXRDen_US
dc.titleAdsorption of lysozyme from aqueous solutions by a novel bentonite-tyrptophane (Bent-Trp) microcomposite affinity sorbenten_US
dc.typeArticleen_US

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