Graphene Oxide Modified Chemically Activated Graphite Electrodes for Detection of microRNA

Basit Öğe Kaydı
dc.contributor.authorErdem, Arzum
dc.contributor.authorEksin, Ece
dc.contributor.authorIsin, Deniz
dc.contributor.authorPolat, Derya
dc.date.accessioned2019-10-27T11:08:26Z
dc.date.available2019-10-27T11:08:26Z
dc.date.issued2017
dc.departmentEge Üniversitesien_US
dc.description.abstractIn our study, graphene oxide (GO) modified graphite electrodes were used for sensitive and selective impedimetric detection of miRNA. After chemical activation of pencil graphite electrode (PGE) surface using covalent agents (CA), GO modification was performed at the surface of chemically activated PGE. Then, CA-GO-PGEs were applied for impedimetric miRNA detection. The microscopic and electrochemical characterization of CA-GO-PGEs was performed by scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS). The optimization of experimental conditions; such as GO concentration, DNA probe concentration and miRNA target concentration was performed by using EIS technique. After the hybridization occurred between miRNA-34a RNA target and its complementary DNA probe, the hybrid was immobilized onto the surface of CA-GO-PGEs. Then, the impedimetric detection of miRNA-DNA hybridization was performed by EIS. The selectivity of our assay was also tested under the optimum experimental conditions.en_US
dc.description.sponsorshipTurkish Scientific and Technological Research Council (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [115Z099]; Turkish Academy of Sciences (TUBA)Turkish Academy of Sciences; TUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [115Z099]en_US
dc.description.sponsorshipArzum Erdem acknowledges the financial support from Turkish Scientific and Technological Research Council (TUBITAK) as the Project Investigator of project (Project No.115Z099) and she also would like to express her gratitude to the Turkish Academy of Sciences (TUBA) as an Principal member for its partial support. Deniz Isin and Derya Polat acknowledges a Project scholarship by TUBITAK (Project No.115Z099).en_US
dc.identifier.doi10.1002/elan.201600761
dc.identifier.endpage1358en_US
dc.identifier.issn1040-0397
dc.identifier.issn1521-4109
dc.identifier.issn1040-0397en_US
dc.identifier.issn1521-4109en_US
dc.identifier.issue5en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1350en_US
dc.identifier.urihttps://doi.org/10.1002/elan.201600761
dc.identifier.urihttps://hdl.handle.net/11454/32168
dc.identifier.volume29en_US
dc.identifier.wosWOS:000401430700019en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherWiley-V C H Verlag Gmbhen_US
dc.relation.ispartofElectroanalysisen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGraphene oxideen_US
dc.subjectmiRNAen_US
dc.subjectnucleic acid hybridizationen_US
dc.subjectpencil graphite electrodeen_US
dc.subjectelectrochemical impedance spectroscopyen_US
dc.titleGraphene Oxide Modified Chemically Activated Graphite Electrodes for Detection of microRNAen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu