High-Yield Biocatalysis of Baicalein 7-O-ß-d-Glucuronide to Baicalein Using Soluble Helix pomatia-Derived ß-Glucuronidase in a Chemically Defined Acidic Medium
| dc.contributor.author | Muderrisoglu C. | |
| dc.contributor.author | Yesil-Celiktas O. | |
| dc.date.accessioned | 2019-10-27T08:01:18Z | |
| dc.date.available | 2019-10-27T08:01:18Z | |
| dc.date.issued | 2019 | |
| dc.department | Ege Üniversitesi | en_US |
| dc.description.abstract | Abstract: Baicalein, showing stronger pharmacological activity, can be obtained by removal of the distal glucuronic acid (GluA) from baicalein 7-O-ß-D-glucuronide (baicalin). In the present study, a chemically defined reaction medium comprised of mildly acidic (pH 4.5, 37 °C) aqueous solution, was formulated for biotransformation of baicalin to baicalein using acidic Helix pomatia derived beta-glucuronidase (HP-GUS), an untested biocatalyst source. The biotransformation was carried out as a batchwise process within an optimised reaction cocktail (with 5% dimethylformamide, v/v) by a 4-h HP-GUS (250 unit/ml) incubation of baicalin (60 ppm) and resulted in a promising conversion ratio of 99% without any by-product formation. The formulated reaction system may offer a novel and efficient alternative for bioproduction of baicalein, which can be vital for pharmaceutical applications. Graphical Abstract: [Figure not available: see fulltext.]. © 2019, Springer Science+Business Media, LLC, part of Springer Nature. | en_US |
| dc.identifier.doi | 10.1007/s10562-019-02745-3 | |
| dc.identifier.issn | 1011372X | |
| dc.identifier.issn | 1011-372X | en_US |
| dc.identifier.scopusquality | Q2 | en_US |
| dc.identifier.uri | https://doi.org/10.1007/s10562-019-02745-3 | |
| dc.identifier.uri | https://hdl.handle.net/11454/24958 | |
| dc.indekslendigikaynak | Scopus | en_US |
| dc.language.iso | en | en_US |
| dc.publisher | Springer New York LLC | en_US |
| dc.relation.ispartof | Catalysis Letters | en_US |
| dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
| dc.rights | info:eu-repo/semantics/closedAccess | en_US |
| dc.subject | Baicalein | en_US |
| dc.subject | Baicalin | en_US |
| dc.subject | Biotransformation | en_US |
| dc.subject | Scutellaria baicalensis Georgi | en_US |
| dc.subject | ß-Glucuronidase | en_US |
| dc.title | High-Yield Biocatalysis of Baicalein 7-O-ß-d-Glucuronide to Baicalein Using Soluble Helix pomatia-Derived ß-Glucuronidase in a Chemically Defined Acidic Medium | en_US |
| dc.type | Article | en_US |
