Plant Growth Regulator Effects on In Vitro Propagation and Stevioside Production in Stevia rebaudiana Bertoni

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dc.contributor.authorRock-Okuyucu, Barbel
dc.contributor.authorBayraktar, Meltem
dc.contributor.authorAkgun, Ismail Hakki
dc.contributor.authorGurel, Aynur
dc.date.accessioned2019-10-27T11:23:38Z
dc.date.available2019-10-27T11:23:38Z
dc.date.issued2016
dc.departmentEge Üniversitesien_US
dc.description.abstractStevia rebaudiana is of great importance due to its steviol glycosides (SGs) which are natural sweeteners used by the food industry as well as having medicinal purposes. In the present study, the effect of plant growth regulators (PGRs) and explant types on in vitro propagation and shoot growth of S. rebaudiana were studied, the effect of PGRs on SGs production was determined. For this purpose, nodal explants and shoot tip explants were cultured on woody plant medium (WPM) supplemented with cytokinins [6-benzyladenine (BA), kinetin (Kn), or thidiazuron (TDZ)] or cytokinins + auxins combinations [BA + indoleacetic acid (IAA); BA + naphthaleneacetic acid (NAA); Kn + IAA; Kn + NAA]. Although, the best shoot proliferationwas obtained onWPMsupplementedwith BA+ NAA combinations, shoots grown on PGR-containing media produced callus at the base of the shoots and showed chlorosis and necrosis. Additionally, shoots showed at all concentrations of TDZ, and at higher concentrations of BA, morphological changes such as malformed leaves and poor shoot growth. In contrast to PGR-containing media, on the PGR-free control medium, the development of shoots and roots occurred simultaneously and healthy and well-developed plantlets were obtained. Thus, we developed an economical viable means of in vitro propagation byminimizing themicropropagation steps and removing the requirement of PGRs. According to the high-performance liquid chromatography (HPLC) results, PGR-free control medium (WPM) led to considerably higher stevioside content in the leaves compared with the PGR(s)-containing media and the highest stevioside [34 mg.g(-1) dry weight (DW)] and rebaudioside A content was only detected on the control medium without PGRs. Steviolbioside, rubusoside, and dulcoside A were detected qualitatively in the leaves of shoots grown on WPM supplemented with 2.27 mu M TDZ, 4.54 mu M TDZ, 2.22 mu M BA + 2.69 mu M NAA, 2.22 mu M BA + 5.37 mu M NAA, 2.32 mu M Kn + 5.71 mu M IAA, or 2.32 mu M Kn + 2.69 mu M NAA.en_US
dc.description.sponsorshipCelal Bayar University Scientific Research Projects CommissionCelal Bayar University [ALS 2008-069]en_US
dc.description.sponsorshipThis research was supported by Celal Bayar University Scientific Research Projects Commission (ALS 2008-069).en_US
dc.identifier.doi10.21273/HORTSCI11093-16
dc.identifier.endpage1580en_US
dc.identifier.issn0018-5345
dc.identifier.issn2327-9834
dc.identifier.issn0018-5345en_US
dc.identifier.issn2327-9834en_US
dc.identifier.issue12en_US
dc.identifier.startpage1573en_US
dc.identifier.urihttps://doi.org/10.21273/HORTSCI11093-16
dc.identifier.urihttps://hdl.handle.net/11454/33195
dc.identifier.volume51en_US
dc.identifier.wosWOS:000392148800022en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherAmer Soc Horticultural Scienceen_US
dc.relation.ispartofHortscienceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectdulcoside Aen_US
dc.subjectHPLCen_US
dc.subjectin vitroen_US
dc.subjectrubusosideen_US
dc.subjectsteviolbiosideen_US
dc.subjectsteviosideen_US
dc.titlePlant Growth Regulator Effects on In Vitro Propagation and Stevioside Production in Stevia rebaudiana Bertonien_US
dc.typeArticleen_US

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