Sensor Platform with a Custom-Tailored Aptamer for Diagnosis of Synthetic Cannabinoids

Basit Öğe Kaydı
dc.contributor.authorBalaban, Simge
dc.contributor.authorMan, Ezgi
dc.contributor.authorDurmus, Ceren
dc.contributor.authorBor, Gulsah
dc.contributor.authorCeylan, Ayse Elcin
dc.contributor.authorGumus, Z. Pinar
dc.contributor.authorTimur, Suna
dc.date.accessioned2020-12-01T12:04:58Z
dc.date.available2020-12-01T12:04:58Z
dc.date.issued2020
dc.departmentEge Üniversitesien_US
dc.description.abstractSynthetic cannabinoids (SCs) are the large group of abused drugs and detection of them is still a challenge. Hence, new methods for analysis of SCs are being investigated. We aimed to develop a novel system for selective analysis of SCs. First, various custom-tailored aptamers against the target SCs were selected through GO-SELEX process. Toggling between different SC analytes during successive rounds of selection was performed to generate cross-reactive aptamers. Then, the amino-capped aptamers were synthesized and easily attached to the cysteamine-covered gold electrodes. Analytical parameters and selectivity of the aptasensors were compared by using electrochemical techniques. After comparison of the analytical features and selectivity towards target analytes, one of the aptamers designated as Apta-1 was chosen for further measurements. the aptasensor was tested by using differential pulse voltammetry technique against JWH-018 (5-pentanoic acid), selected as a model for SCs. the linearity and limit of detection were determined as 0.01-1.0 ng/mL and 0.036 ng/mL. Finally, sample application in synthetic urine samples was successfully performed with standard addition method, as confirmed by LC-QTOF/MS. JWH-018 (4-hydroxypentyl), JWH-073 (3-hydroxybutyl), JWH-250 (5-hidroxypentyl) and HU-210 were used to test the selectivity of the aptasensor and the system was shown to recognize all these SCs. Also other illegal drugs did not significantly interfere with the signal responses.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [117Z152]; Republic of Turkey, Ministry of DevelopmentTurkiye Cumhuriyeti Kalkinma Bakanligi [2016K121190]; Ege University Central Research Testing and Analysis Laboratory Research and Application Center (EGE-MATAL)en_US
dc.description.sponsorshipThis study was supported by the Scientific and Technological Research Council of Turkey (TUBITAK, Project Grant No: 117Z152). This work was also supported by Republic of Turkey, Ministry of Development (Project Grant No: 2016K121190) and Ege University Central Research Testing and Analysis Laboratory Research and Application Center (EGE-MATAL) chromatography laboratories were used in this study.en_US
dc.identifier.doi10.1002/elan.201900670
dc.identifier.endpage665en_US
dc.identifier.issn1040-0397
dc.identifier.issn1521-4109
dc.identifier.issn1040-0397en_US
dc.identifier.issn1521-4109en_US
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85078064608en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage656en_US
dc.identifier.urihttps://doi.org/10.1002/elan.201900670
dc.identifier.urihttps://hdl.handle.net/11454/62829
dc.identifier.volume32en_US
dc.identifier.wosWOS:000505868000001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherWiley-V C H Verlag Gmbhen_US
dc.relation.ispartofElectroanalysisen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCustom-tailored aptameren_US
dc.subjectGO-SELEXen_US
dc.subjectAptasensoren_US
dc.subjectSynthetic cannabinoidsen_US
dc.subjectJWH-018 (5-pentanoic acid metabolite)en_US
dc.titleSensor Platform with a Custom-Tailored Aptamer for Diagnosis of Synthetic Cannabinoidsen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu