Modelling coupled dynamics of diffusive–convective mass transfer in a microfluidic device and determination of hydrodynamic dispersion coefficient

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dc.contributor.authorYildiz-Ozturk E.
dc.contributor.authorYucel M.
dc.contributor.authorMuderrisoglu C.
dc.contributor.authorSargin S.
dc.contributor.authorYesil-Celiktas O.
dc.date.accessioned2019-10-27T08:03:25Z
dc.date.available2019-10-27T08:03:25Z
dc.date.issued2017
dc.departmentEge Üniversitesien_US
dc.description.abstractOne of the challenges in mathematical modelling of microchips is the lack of available data for dispersion coefficients of biomolecules. The main focus of this study was to determine the hydrodnamic dispersion coefficients of the model substrates, 4-Nitrophenyl-ß-D-glucopyranoside (pNPG_1) and 4-Nitrophenyl-ß-D-glucuronide (pNPG_2) for ß-glucosidase and ß-glucoronidase. The substrate solutions were pumped through the silica porous gel inside the S-shaped PDMS microreactor at flow rates of 1, 3 and 5 µl/min. The output flow was collected with respect to time and quantified by UPLC. The general mathematical model was derived for the coupled dynamics of convective–diffusive mass transfer and a computational algorithm was developed for the numerical solutions of the derived partial differential equations in MATLAB. The hydrodynamic dispersion coefficients of pNPG_1 were determined as 0.370 × 10-6, 3.638 × 10-6 and 11.680 × 10-6 m2/s, while as 0.368 × 10-6, 1.515 × 10-6and 3.503 × 10-6m2/s for pNPG_2 at respective flow rates. Furthermore, the relations between dispersion coefficients and flow rates were investigated. Obtained hydrodynamic dispersion coefficients can be used for modelling of pNPG reactions which may also be adapted to other enzyme related reactions within life sciences. © 2017 Taiwan Institute of Chemical Engineersen_US
dc.description.sponsorship113M050en_US
dc.description.sponsorshipThe research support by the Scientific and Technical Research Council of Turkey ( TUBITAK ) ( 113M050 ) is highly appreciated. --en_US
dc.identifier.doi10.1016/j.jtice.2017.08.033
dc.identifier.endpage106en_US
dc.identifier.issn1876-1070
dc.identifier.issn1876-1070en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage100en_US
dc.identifier.urihttps://doi.org/10.1016/j.jtice.2017.08.033
dc.identifier.urihttps://hdl.handle.net/11454/25354
dc.identifier.volume80en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherTaiwan Institute of Chemical Engineersen_US
dc.relation.ispartofJournal of the Taiwan Institute of Chemical Engineersen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectHydrodynamic dispersion coefficienten_US
dc.subjectLag-time analysisen_US
dc.subjectMicrofluidicsen_US
dc.subjectModellingen_US
dc.subjectPorous silica gelen_US
dc.subjectSimulationen_US
dc.titleModelling coupled dynamics of diffusive–convective mass transfer in a microfluidic device and determination of hydrodynamic dispersion coefficienten_US
dc.typeArticleen_US

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