Cytotoxicity in astrocyte cultures due to pH changes and protection by glutathione [Astrosit hücre kültürlerinde pH degiş ikliginin yarattigi toksisite ve glutatyonun koruyucu etkisi]

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dc.contributor.authorYilmaz O.
dc.contributor.authorTaskiran D.
dc.date.accessioned2019-10-26T22:37:34Z
dc.date.available2019-10-26T22:37:34Z
dc.date.issued2010
dc.departmentEge Üniversitesien_US
dc.description.abstractBackground: It is well known that extracellular and intracellular pH changes have cytotoxic effects on astrocytes and neurons. In the present study, we aimed to evaluate the cytotoxic effects of acidosis on astrocyte cultures by measuring cell death and oxidative stress parameters. We also investigated the protective effects of ascorbic acid and gluthatione as antioxidants against toxicity due to pH changes in the cultures. Materials and Methods: Primary astrocyte cultures were prepared from 1-2 day old neonatal rats. Astrocytes were incubated in media with or without pH 5,5 ± 0,1 for 2 h and treated with ascorbic acid (AA, 1 mM) and glutathione (GSH, 1 mM). The effects of acidosis and antioxidants were assessed by measuring lactate dehydrogenase (LDH), superoxide dismutase (SOD) and catalase activities and nitrite levels in the cultures. Results: LDH activity, nitrite levels and antioxidant enzyme activities were found significantly elevated in the acidosis group than the control (p< 0.0005). Treatment of the astrocytes with GSH decreased cell death, nitrite levels and antioxidant enzyme activities compare to the acidosis group. Although the treatment of the cultures with AA lowered antioxidant enzyme activities and ntirite levels, there was no protective effect on astrocyte viability. Conclusions: Our results demonstrated that decreased of pH levels may effect astrocyte viability and antioxidant enzyme activities. Treatment of the astrocytes with GSH may protect them from cytotoxicity and oxidative stress. Overall, these results support that acidosis and oxidative stress closely interact with each other and may trigger cell damage in brain.en_US
dc.identifier.endpage68en_US
dc.identifier.issn1300-1817
dc.identifier.issn1300-1817en_US
dc.identifier.issue1en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage61en_US
dc.identifier.urihttps://hdl.handle.net/11454/19910
dc.identifier.volume27en_US
dc.indekslendigikaynakScopusen_US
dc.language.isotren_US
dc.relation.ispartofJournal of Neurological Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAcidosisen_US
dc.subjectAstrocyteen_US
dc.subjectGlutathioneen_US
dc.subjectOxidative stressen_US
dc.subjectToxicityen_US
dc.titleCytotoxicity in astrocyte cultures due to pH changes and protection by glutathione [Astrosit hücre kültürlerinde pH degiş ikliginin yarattigi toksisite ve glutatyonun koruyucu etkisi]en_US
dc.typeArticleen_US

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