Laccase assay based on electrochemistry and fluorescence detection via anthracene sequestered poly(amic acid) films

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dc.contributor.authorEsen, Elif
dc.contributor.authorYazgan, Idris
dc.contributor.authorDemirkol, Dilek Odaci
dc.contributor.authorTimur, Suna
dc.date.accessioned2019-10-27T10:01:33Z
dc.date.available2019-10-27T10:01:33Z
dc.date.issued2018
dc.departmentEge Üniversitesien_US
dc.description.abstractPhenols are among the most problematic organic compounds, which manipulates metabolism of living organisms and pose threat to environment. Detection of total phenol at the lowest allowable concentration is critical, so development of sensitive and easy to use methods have been heavily investigated. Hereby, for the first time, anthracene (Ant) was sequestered into poly(amic acid) (PAA) to form fluorescent and conductive film support for laccase (Lac) immobilization to quantify total phenol content at high specificity and selectivity. Ant-PAA/Lac films were applied to fluorescent and electrochemical quantification of phenol, which provided high sensitivity (LOD 46 mu M) and reproducibility (RSD, 0.5%) for the tested 10 phenolic compounds including phenol, catechol, 3-acetominophenol, hydroquinone, L-DOPA, 2,6-dimethylphenyl, syringalzadine, 3,5-dimethoxy-4-hydroxycinnamic acid, 2,5-dimethoxyaniline and guaiacol. The sensor system showed strong rejection to common organic interferent while real sample application revealed the applicability of the sensor. Therefore, the results can call the sensor system, particularly fluorescence-based quantification in 96-well plate, is an alternative approach for high-throughput screening of total phenolic compounds in waste-water at cost-effective manner.en_US
dc.description.sponsorshipBAGEP Award of the Science Academy; Ege University Research FoundationEge University [2017FEN012]en_US
dc.description.sponsorshipThis work was supported by the BAGEP Award of the Science Academy. Authors thank to Ege University Research Foundation (Project no: 2017FEN012) for partial support.en_US
dc.identifier.doi10.1016/j.reactfunctpolym.2018.07.001
dc.identifier.endpage43en_US
dc.identifier.issn1381-5148
dc.identifier.issn1873-166X
dc.identifier.issn1381-5148en_US
dc.identifier.issn1873-166Xen_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage36en_US
dc.identifier.urihttps://doi.org/10.1016/j.reactfunctpolym.2018.07.001
dc.identifier.urihttps://hdl.handle.net/11454/29867
dc.identifier.volume131en_US
dc.identifier.wosWOS:000448225200005en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofReactive & Functional Polymersen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPhenol detectionen_US
dc.subjectPoly (amic acid) filmsen_US
dc.subjectFluorescence sensingen_US
dc.subjectElectrochemical sensingen_US
dc.subjectLaccaseen_US
dc.titleLaccase assay based on electrochemistry and fluorescence detection via anthracene sequestered poly(amic acid) filmsen_US
dc.typeArticleen_US

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