Amine-intercalated montmorillonite matrices for enzyme immobilization and biosensing applications

Basit Öğe Kaydı
dc.contributor.authorSeleci, Muharrem
dc.contributor.authorAg, Didem
dc.contributor.authorYalcinkaya, Esra Evrim
dc.contributor.authorDemirkol, Dilek Odaci
dc.contributor.authorGuler, Cetin
dc.contributor.authorTimur, Suna
dc.date.accessioned2019-10-27T21:34:37Z
dc.date.available2019-10-27T21:34:37Z
dc.date.issued2012
dc.departmentEge Üniversitesien_US
dc.description.abstractClay based biosensors were developed using montmorillonite (Mont) modified with methyl (M) and dimethylamine (DM). X-ray diffraction, Fourier transform infrared spectroscopy, zeta potential and thermal gravimetric measurements were used to characterize the modified clays. After immobilization of glucose oxidase (GOx) via clay on the glassy carbon electrode, its application as a glucose biosensor was investigated in detail. The best response characteristics were obtained by DM-Mont and optimization of enzyme amount, reproducibility of biosensor fabrication, repeatability of measurements and operational stability were all evaluated. The optimized biosensor showed a very good linearity between 0.05 mM and 1.0 mM, a 7 s response time and a limit of detection to glucose of 0.038 mM. Also, kinetic parameters and stabilities were determined. Apparent K-m and I-max values were found as 0.73 mM and 2.955 mu A, respectively. As well as batch configuration, the DM-Mont/GOx biosensor was successfully applied in the flow injection analysis mode. Finally, the performance of the DM-Mont/GOx biosensor to analyze glucose in a wine sample was compared with HPLC.en_US
dc.identifier.doi10.1039/c2ra01225a
dc.identifier.endpage2118en_US
dc.identifier.issn2046-2069
dc.identifier.issn2046-2069en_US
dc.identifier.issue5en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage2112en_US
dc.identifier.urihttps://doi.org/10.1039/c2ra01225a
dc.identifier.urihttps://hdl.handle.net/11454/45642
dc.identifier.volume2en_US
dc.identifier.wosWOS:000300317700056en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherRoyal Soc Chemistryen_US
dc.relation.ispartofRsc Advancesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.titleAmine-intercalated montmorillonite matrices for enzyme immobilization and biosensing applicationsen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu