Characteristics of various cell types in primary insect cell culture from larval ovaries of silkworm (Bombyx mori L.)

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dc.contributor.authorSoya S.
dc.contributor.authorCan H.
dc.date.accessioned2019-10-26T21:12:24Z
dc.date.available2019-10-26T21:12:24Z
dc.date.issued2019
dc.departmentEge Üniversitesien_US
dc.description.abstractAs insect cell lines can be used in baculovirus expression systems for producing recombinant proteins, there is a need to establish new cell lines. Insect cell lines are thought to be useful in production of humanized recombinant proteins. Hence, we aimed to establish a culture from larval ovaries of Bombyx mori to investigate the cell characteristics and growth properties. Two sets of primary cell cultures were established from ovaries of fifth instar larvae. Characterization of cell line was carried out by comparing the PCR products of cell line and silkworms from COI (cytochrome c oxidase subunit I) specific primers. In the first set of cultures, the culture population was found to be heterogeneous and cell sizes were variable. Fibroblastic, epithelial-like, spindle-like, and round cells were observed after 7 th day. Interestingly, contractions were observed in muscle-like cells at the end of first month, and stayed stable until the fourth month. At the beginning of the fourth month, because cell death increased significantly, cell concentrations decreased. In the second set of cultures, it was determined that doubling time was about 72 h with high viability and confluency of cells were higher in early passages. PCR which was conducted for characterization of cells resulted in a 710 bp product in each cell line and silkworms. In conclusion, growth rate detected in this study was observed to be compatible with literature. Also, it was thought that if primary culture is supported with growth stimulators, growth rate can be more accelerated and thi s primary culture can be used more efficiently in recombinant protein production studies. © 2019, Pakistan Agricultural Scientists Forum. All rights reserved.en_US
dc.identifier.endpage332en_US
dc.identifier.issn1018-7081
dc.identifier.issn1018-7081en_US
dc.identifier.issue1en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage325en_US
dc.identifier.urihttps://hdl.handle.net/11454/15691
dc.identifier.volume29en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherPakistan Agricultural Scientists Forumen_US
dc.relation.ispartofJournal of Animal and Plant Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBombyx morien_US
dc.subjectCell morphologyen_US
dc.subjectCharacterizationen_US
dc.subjectGrowth analysisen_US
dc.subjectOvaryen_US
dc.subjectPrimary cell cultureen_US
dc.titleCharacteristics of various cell types in primary insect cell culture from larval ovaries of silkworm (Bombyx mori L.)en_US
dc.typeArticleen_US

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