Screening of cytotoxicity and DNA topoisomerase Ha inhibitory activity of Turkish Onosma species

Basit Öğe Kaydı
dc.authoridBedir, Erdal/0000-0003-1262-063X
dc.authoridPIRHAN, Ademi Fahri/0000-0001-9368-2343
dc.authoridKARAKOYUN, Cigdem/0000-0003-1150-7061
dc.authoridguzel, ozge/0000-0003-4224-5826
dc.authorscopusid57766377200
dc.authorscopusid57201676551
dc.authorscopusid57199168471
dc.authorscopusid57203016888
dc.authorscopusid57764869400
dc.authorscopusid37026890100
dc.authorscopusid7003998497
dc.authorwosidKARAKOYUN, Çiğdem/GZG-3522-2022
dc.contributor.authorGuzel, Ozge
dc.contributor.authorDuman, Seda
dc.contributor.authorYilmaz, Sinem
dc.contributor.authorKarakoyun, Cigdem
dc.contributor.authorKul, Demet
dc.contributor.authorPirhan, Ademi Fahri
dc.contributor.authorBedir, Erdal
dc.date.accessioned2023-01-12T20:16:02Z
dc.date.available2023-01-12T20:16:02Z
dc.date.issued2021
dc.departmentN/A/Departmenten_US
dc.description.abstractOnosma L., the largest genus of Boraginaceae, is represented by 105 species in Turkey with an endemism rate of 52%. Phytochemical studies indicate that Boraginaceae plants mainly comprise naphthoquinones with a wide range of biological activities including anticancer, antiinflammatory, wound healing, and antioxidant effects. However, few taxa of the genus Onosma have been investigated in detail for their bioactivities. Considering the high rate of endemism and an inadequate number of bioactivity screening studies in literature, we aimed to evaluate the cytotoxic effects and topoisomerase inhibitory activities of some Onosma species growing in southwestern Turkey. Here, we describe a comprehensive cytotoxic activity screening study on petroleum ether, dichloromethane, and methanol extracts of the roots of 20 identified and one unidentified Onosma taxa. The MTT cell viability assay has been performed to investigate the cytotoxicity of the extracts against seven cancer cell lines (MCF-7, HeLa > Hep G2, A549, Capan-1, HCC-1937, and DU-145) and a noncancerous cell line (MRC-5), while doxorubicin was served as a positive standard. The petroleum ether extracts of O. aksoyii Aytac&Turkmen, O. isaurica Boiss. and Heldr., O. taurica Pallas ex Willd. var. taurica and O. alborosea Fisch. & C.A. Mey subsp. alborosea var. alborosea were determined as the most active ones based on their IC50 values. DNA topoisomerase Ila inhibition assay was conducted on the petroleum ether and dichloromethane extracts of these four active species, and almost all tested extracts demonstrated strong inhibition on the enzyme at a concentration of 0.1 mg/mL. Our cytotoxicity screening results were consistent with the findings of the topoisomerase Ila inhibition test. This study advocates the significant role of Onosma species in the field of anticancer drug discovery.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TuBTAK) [116Z463]en_US
dc.description.sponsorshipThis project was supported by The Scientific and Technological Research Council of Turkey (TuBTAK, Project No: 116Z463) . We are very grateful to Prof. Petek Ballar Krmzbayrak for opening her laboratory for cytotoxicity screening studies. We also thank Dr. Sevil Zencir and Prof. Zeki Topcu for their contributions to topoisomerases assays.en_US
dc.identifier.doi10.3906/bot-2108-23
dc.identifier.endpage788en_US
dc.identifier.issn1300-008X
dc.identifier.issn1303-6106
dc.identifier.issn1300-008Xen_US
dc.identifier.issn1303-6106en_US
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-85132845626en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage776en_US
dc.identifier.urihttps://doi.org/10.3906/bot-2108-23
dc.identifier.urihttps://hdl.handle.net/11454/78631
dc.identifier.volume45en_US
dc.identifier.wosWOS:000739936300006en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherTubitak Scientific & Technical Research Council Turkeyen_US
dc.relation.ispartofTurkish Journal Of Botanyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectOnosmaen_US
dc.subjectendemicen_US
dc.subjectcytotoxicen_US
dc.subjectbioactivityen_US
dc.subjectscreeningen_US
dc.subjecttopoisomeraseen_US
dc.subjectIi-Alpha Expressionen_US
dc.subjectNaphthazarin Derivativesen_US
dc.subjectShikoninen_US
dc.subjectCanceren_US
dc.subjectNaphthoquinonesen_US
dc.subjectBoraginaceaeen_US
dc.subjectMechanismen_US
dc.subjectApoptosisen_US
dc.subjectAssayen_US
dc.titleScreening of cytotoxicity and DNA topoisomerase Ha inhibitory activity of Turkish Onosma speciesen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu