A bio-imprinted ascorbate oxidase biosensor
Küçük Resim Yok
Tarih
2007
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Taylor & Francis Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Interest in bio-imprinting techniques has increased because it allows some stability characteristics of enzymes to be improved. In this study, we developed a simple way to improve the thermal and pH stabilities of ascorbate oxidase biosensor. The membrane of a Clark oxygen electrode was coated by a bioactive layer containing ascorbate oxidase and gelatin cross-linked by glutaraldehyde. Citrate was used to imprint the ascorbate oxidase molecularly. The optimum temperature and pH of both unmodified and citrate modified biosensors were investigated, by comparing their resulting stability. Also, calibration graphs and operational stabilities were compared with each other. The results showed that this simple way should be used to improve the stabilities of a biosensor.
Açıklama
7th Workshop on Biosensors and BioAnalytical Mu-Techniques for Environmental and Clinical Analysis -- SEP 10-14, 2006 -- Kusadasi, TURKEY
Anahtar Kelimeler
MIP biosensor, ascorbate oxidase, biosensor stability, stability improvement, bio-imprinting
Kaynak
International Journal of Environmental Analytical Chemistry
WoS Q Değeri
Q3
Scopus Q Değeri
Q3
Cilt
87
Sayı
10.Nov
