Lung carcinoma spheroids embedded in a microfluidic platform

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dc.contributor.authorYildiz-Ozturk E.
dc.contributor.authorSaglam-Metiner P.
dc.contributor.authorYesil-Celiktas O.
dc.date.accessioned2021-05-03T20:58:58Z
dc.date.available2021-05-03T20:58:58Z
dc.date.issued2021
dc.description.abstractThree-dimensional (3D) spheroid cell cultures are excellent models used in cancer biology research and drug screening. The objective of this study was to develop a lung carcinoma spheroid based microfluidic platform with perfusion function to mimic lung cancer pathology and investigate the effect of a potential drug molecule, panaxatriol. Spheroids were successfully formed on agar microtissue molds at the end of 10 days, reaching an average diameter of about 317.18 ± 4.05 ?m and subsequently transferred to 3D dynamic microfluidic system with perfusion function. While the size of the 3D spheroids embedded in the Matrigel matrix in the platform had gradually increased both in the static and dynamic control groups, the size of the spheroids were reduced and fragmented in the drug treated groups. Cell viability results showed that panaxatriol exhibited higher cytotoxic effect on cancer cells than healthy cells and the IC50 value was determined as 61.55 µM. Furthermore, panaxatriol has been more effective on single cells around the spheroid structure, whereas less in 3D spheroid tissues with a compact structure in static conditions compared to dynamic systems, where a flow rate of 2 µL/min leading to a shear stress of 0.002 dyne/cm2 was applied. Application of such dynamic systems will contribute to advancing basic research and increasing the predictive accuracy of potential drug molecules, which may accelerate the translation of novel therapeutics to the clinic, possibly decreasing the use of animal models. Graphic abstract: [Figure not available: see fulltext.] © 2021, The Author(s), under exclusive licence to Springer Nature B.V.en_US
dc.description.sponsorship113M050 Türkiye Bilimsel ve Teknolojik Araştirma Kurumu, TÜBITAKen_US
dc.description.sponsorshipThe financial support provided by the Scientific and Technological Research Council of Turkey (TUBITAK) through 113M050 Grant is highly appreciated.en_US
dc.identifier.doi10.1007/s10616-021-00470-7
dc.identifier.issn0920-9069
dc.identifier.issn0920-9069en_US
dc.identifier.scopus2-s2.0-85104633558en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.urihttps://doi.org/10.1007/s10616-021-00470-7
dc.identifier.urihttps://hdl.handle.net/11454/71509
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringer Science and Business Media B.V.en_US
dc.relation.ispartofCytotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subject3D spheroid cultureen_US
dc.subjectCytotoxicityen_US
dc.subjectLung carcinomaen_US
dc.subjectMicrofluidic systemen_US
dc.subjectPanaxatriolen_US
dc.titleLung carcinoma spheroids embedded in a microfluidic platformen_US
dc.typeArticleen_US

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