Multiplexed aptasensor for detection of acute myocardial infraction (AMI) biomarkers

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dc.authoridMani, Veerappan/0000-0002-0756-7398
dc.authoridDemirci, Ferhat/0000-0002-5999-3399
dc.authoridnaneva, stela/0000-0002-8993-674X
dc.authoridsalama, khaled Nabil/0000-0001-7742-1282
dc.contributor.authorBeduk, Duygu
dc.contributor.authorBeduk, Tutku
dc.contributor.authorLahcen, Abdellatif Ait
dc.contributor.authorMani, Veerappan
dc.contributor.authorCelik, Emine Guler
dc.contributor.authorIskenderoglu, Gamze
dc.contributor.authorDemirci, Ferhat
dc.date.accessioned2024-08-31T07:47:33Z
dc.date.available2024-08-31T07:47:33Z
dc.date.issued2024
dc.departmentEge Üniversitesien_US
dc.description.abstractAcute myocardial infarction (AMI) is a leading global cause of death. Diagnosis is challenging as cardiac biomarkers are only detectable for a few hours after AMI onset, and current methods are time-consuming and lack selectivity. Therefore, multiple immunological test systems have great importance for rapid and accurate diagnosis. In this context, we developed a rapid immunodiagnostic sensor platform for simultaneous electrochemical detection of cardiac troponin T (cTnT), troponin I (cTnI), and C-reactive protein (CRP) using nanostructured gold-modified laser-scribed graphene (LSG). Aptamer sensors were integrated into the LSG platform for selective AMI biomarkers sensing. Clinical validation was performed on biomarkers from blood samples of 51 AMI patients and 9 healthy controls. Limits of detection were 1.65 ng mL-1 cTnT, 2.58 ng mL-1 cTnI, and 1.84 ng mL-1 CRP. The analytical results determined by the developed platform were compared with the routine standard values of the same patients to prove the accuracy of aptasensors. Sensor results agreed well with standard laboratory assays, highlighting the accuracy of the test platform. The cTnT, cTnI and CRP multiplexed sensor platform demonstrates excellent performance for rapid and sensitive AMI screening. Schematic illustration of the developed aptasensor for multiplex detection of AMI biomarkers.en_US
dc.description.sponsorshipEge niversitesi; King Abdullah University of Science and Technology (KAUST) Saudi Arabia; KAUST Smart-Health Initiativeen_US
dc.description.sponsorshipThe authors would like to thank the Ege University Central Research Testing and Analysis Laboratory Application and Research Center (EGE-MATAL) for providing the equipment; Ege University; and King Abdullah University of Science and Technology (KAUST) Saudi Arabia and KAUST Smart-Health Initiative for the financial support.en_US
dc.identifier.doi10.1039/d4sd00010b
dc.identifier.endpage1027en_US
dc.identifier.issn2635-0998
dc.identifier.issue6en_US
dc.identifier.scopus2-s2.0-85192439941en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage1020en_US
dc.identifier.urihttps://doi.org/10.1039/d4sd00010b
dc.identifier.urihttps://hdl.handle.net/11454/104470
dc.identifier.volume3en_US
dc.identifier.wosWOS:001216694900001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherRoyal Soc Chemistryen_US
dc.relation.ispartofSensors & Diagnosticsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240831_Uen_US
dc.subjectCardiac Biomarkersen_US
dc.subjectBiosensorsen_US
dc.titleMultiplexed aptasensor for detection of acute myocardial infraction (AMI) biomarkersen_US
dc.typeArticleen_US

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