Dendrimer modified 8-channel screen-printed electrochemical array system for impedimetric detection of activated protein C

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dc.contributor.authorErdem, Arzum
dc.contributor.authorCongur, Gulsah
dc.date.accessioned2019-10-27T22:14:56Z
dc.date.available2019-10-27T22:14:56Z
dc.date.issued2014
dc.departmentEge Üniversitesien_US
dc.description.abstractThe 8-channel screen-printed electrochemical array system (MULTI-SPE8) was developed as an impedimetric aptasensor, and applied for monitoring the interaction between DNA aptamer (DNA-APT) and its cognate protein, human activated protein C (APC), which is the key enzyme of the protein C pathway. Poly(amidoamine) (PAMAM) dendrimer having 16 succinamic acid surface groups (generation 2, G2-PS) was utilized in order to modify the surface of each carbon-based working electrode in MULTI-SPE8, and accordingly, an enhanced sensor response was recorded. Amino linked DNA-APT was then immobilized onto the surface of G2-PS/MULTI-SPE8, and its interaction with APC was explored. After the optimization of the experimental conditions; such as G2-PS, DNA-APT and APC concentration, the selectivity of the electrochemical aptasensor array system was tested in the presence of numerous biomolecules: protein C (PC), thrombin (THR), bovine serum albumin (BSA), factor Va (FVa) and chromogenic substrate (KS) in buffer media, or in the artificial serum: fetal bovine serum (FBS). The dendrimer-modified aptasensor technology based on MULTI-SPE8 has several advantages, such as disposable, fast screening of analyte at eight channels in one batch with low cost per measurement and resulting in a sensitive and selective indirect method for the analysis of APC, with the detection limits of 1.81 mu g/mL (0.64 pmol in 20 mu L sample) in buffer solution and 0.02 mu g/mL (8.22 fmol in 20 mu L sample) in diluted FBS. (C) 2014 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipTurkish Scientific and Technological Research Council (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [111T073]; Turkish Academy of Sciences (TUBA)Turkish Academy of Sciencesen_US
dc.description.sponsorshipAE acknowledges the financial support from Turkish Scientific and Technological Research Council (TUBITAK; Project no. 111T073), and she also expresses her gratitude to the Turkish Academy of Sciences (TUBA) as an associate member for its partial support. GC acknowledges a master project scholarship through project (TUBITAK Project no. 111T073). The authors also thank Prof. Gunter Mayer for his valuable scientific comments during this study.en_US
dc.identifier.doi10.1016/j.snb.2014.01.103
dc.identifier.endpage174en_US
dc.identifier.issn0925-4005
dc.identifier.issn0925-4005en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage168en_US
dc.identifier.urihttps://doi.org/10.1016/j.snb.2014.01.103
dc.identifier.urihttps://hdl.handle.net/11454/50094
dc.identifier.volume196en_US
dc.identifier.wosWOS:000333652400023en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevier Science Saen_US
dc.relation.ispartofSensors and Actuators B-Chemicalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPAMAM dendrimeren_US
dc.subject8-channel screen printed electrochemical array systemen_US
dc.subjectElectrochemical impedance spectroscopy (EIS)en_US
dc.subjectActivated protein C (APC)en_US
dc.subjectDNA aptameren_US
dc.titleDendrimer modified 8-channel screen-printed electrochemical array system for impedimetric detection of activated protein Cen_US
dc.typeArticleen_US

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