Melatonin levels and microRNA (miRNA) relative expression profile in the follicular ambient microenvironment in patients undergoing in vitro fertilization process

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dc.contributor.authorKhan, H.L.
dc.contributor.authorBhatti, S.
dc.contributor.authorAbbas, S.
dc.contributor.authorKaloglu, C.
dc.contributor.authorQurat-ul-Ain Zahra, S.
dc.contributor.authorKhan, Y.L.
dc.contributor.authorAydin, H.H.
dc.date.accessioned2020-12-01T11:53:06Z
dc.date.available2020-12-01T11:53:06Z
dc.date.issued2020
dc.departmentEge Üniversitesien_US
dc.description.abstractPurpose: Intrafollicular fluid (IFF) melatonin plays a decisive role in maintaining granulosa cells’ DNA integrity and protects them against apoptosis. It reduces oxidative stress and improves the oocyte quality with a higher fertilization rate. Method: This prospective study investigated the antioxidant property of IFF melatonin and its impact on IVF outcome parameters. We also explored the relative expression of five microRNAs (miR-663b, miR-320a, miR-766-3p, miR-132-3p, miR-16-5p) and levels of cell-free DNA (cfDNA) by real-time PCR in unexplained infertile patients. We collected 425 follicular fluid (FF) samples containing mature oocytes from 295 patients undergoing IVF. Results: Patients were subgrouped based on IFF melatonin concentration (group A ? 30 pg/mL, group B > 70 to ? 110 pg/mL, group C > 111 to ? 385 pg/mL). Our results showed that patients with ? 30 pg/mL IFF melatonin levels have significantly higher oxidative stress markers, cfDNA levels, and lower relative expression of miR-663b, miR-320a, miR-766-3p, miR-132-3p, and miR-16-5p compared to other subgroups (p < 0.001). Similarly, they have a low fertilization rate and a reduced number of high-quality day 3 embryos. Conclusion: Findings suggest that the therapeutic use of melatonin produces a considerable rise in the number of mature oocytes retrieved, fertilization rate, and good-quality embryo selection. Furthermore, miRNA signature enhances the quality of embryo selection, thus, may allow us to classify them as non-invasive biomarkers to identify good-quality embryos. © 2020, Springer Science+Business Media, LLC, part of Springer Nature.en_US
dc.identifier.doi10.1007/s10815-020-02010-2
dc.identifier.issn1058-0468
dc.identifier.issn1058-0468en_US
dc.identifier.pmid33226531en_US
dc.identifier.scopus2-s2.0-85096441933en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1007/s10815-020-02010-2
dc.identifier.urihttps://hdl.handle.net/11454/61736
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofJournal of Assisted Reproduction and Geneticsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCell-free DNAen_US
dc.subjectEmbryo qualityen_US
dc.subjectFollicular fluiden_US
dc.subjectMelatoninen_US
dc.subjectmiRNA expressionen_US
dc.subjectUnexplained infertilityen_US
dc.titleMelatonin levels and microRNA (miRNA) relative expression profile in the follicular ambient microenvironment in patients undergoing in vitro fertilization processen_US
dc.typeArticleen_US

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