Evaluation of Fis-1 and miR-484 Expression Levels in Tumor Tissue Samples and Healthy Tumor Margins in Lung Cancer

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dc.authoridzarredar, habib/0000-0002-7217-7521
dc.contributor.authorNamvar, Leila
dc.contributor.authorNadiri, Mehdi
dc.contributor.authorMohebbi, Zahra
dc.contributor.authorAsadi, Milad
dc.contributor.authorSeyyedi, Maryam
dc.contributor.authorAmiri-Sadeghan, Amir
dc.contributor.authorSoleimani, Zahra
dc.date.accessioned2024-08-31T07:49:42Z
dc.date.available2024-08-31T07:49:42Z
dc.date.issued2022
dc.departmentEge Üniversitesien_US
dc.description.abstractBackground: MicroRNAs (miRNAs) regulate gene expression and various cellular activities. They also hold significant importance in the progression and development of human malignancies. Among these, miRNA-484 and the Fis-1 gene have been identified as having substantial roles in lung cancer. This study aims to ascertain miRNA-484 and Fis-1 gene expression levels in non-small cell lung cancer (NSCLC) patients.Method: In this case-control study, 45 pairs of tumor tissues and their corresponding healthy margin tissues were surgically obtained from NSCLC patients and promptly preserved in liquid nitrogen after excision. Total RNA extraction was performed using TRIzol, followed by cDNA synthesis using a designated kit. Afterward, we used quantitative reverse transcription polymerase chain reaction (qRT-PCR) to measure the expression levels of miRNA-484 and the Fis-1 gene. Furthermore, the clinico-pathological characteristics of the NSCLC patients were assessed.Results: Our findings revealed an upregulation of miRNA-484 expression and downregulation of Fis-1 gene expression in NSCLC tissues compared with non-tumor tissues. Additionally, significant correlations were observed between miRNA-484 and Fis-1 gene expression levels and clinicopathological features of the patients, including factors such as lymph node involvement and distant metastasis.Conclusion: These findings suggest the potential utility of Fis-1 and miR-484 as prognostic and diagnostic markers in NSCLC.en_US
dc.identifier.doi10.30476/mejc.2023.96826.1839
dc.identifier.endpage7en_US
dc.identifier.issn2008-6709
dc.identifier.issn2008-6687
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-85181211505en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage1en_US
dc.identifier.urihttps://doi.org/10.30476/mejc.2023.96826.1839
dc.identifier.urihttps://hdl.handle.net/11454/104964
dc.identifier.volume15en_US
dc.identifier.wosWOS:001134573600002en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherShiraz Univ Medical Sciencesen_US
dc.relation.ispartofMiddle East Journal of Canceren_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmz20240831_Uen_US
dc.subjectMirna-486en_US
dc.subjectFis-1 Proteinen_US
dc.subjectCarcinomaen_US
dc.subjectNon-Small-Cell Lungen_US
dc.subjectMalignancyen_US
dc.titleEvaluation of Fis-1 and miR-484 Expression Levels in Tumor Tissue Samples and Healthy Tumor Margins in Lung Canceren_US
dc.typeArticleen_US

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