Amine intercalated clay surfaces for microbial cell immobilization and biosensing applications

dc.contributor.authorDemir, Bilal
dc.contributor.authorSeleci, Muharrem
dc.contributor.authorAg, Didem
dc.contributor.authorCevik, Serdar
dc.contributor.authorYalcinkaya, Esra Evrim
dc.contributor.authorDemirkol, Dilek O.
dc.contributor.authorAnik, Ulku
dc.contributor.authorTimur, Suna
dc.date.accessioned2019-10-27T21:43:19Z
dc.date.available2019-10-27T21:43:19Z
dc.date.issued2013
dc.departmentEge Üniversitesien_US
dc.description.abstractTrimethylamine (TM) intercalated montmorillonite (Mont) clay was prepared and characterized using X-ray diffraction, Fourier transform infrared spectroscopy, zeta potential and thermal gravimetric measurements. Gluconobacter oxydans cells were immobilized on the micro-structured matrix and used in microbial sensor applications. Sensor responses were based on the respiratory activity of the cells and the consumption of oxygen was monitored at 20.7 V (vs. Ag/AgCl and platinum electrodes) by using glucose as a substrate. Stabilization of the bacteria was performed on the Mont using glutaraldehyde. Optimization of the TM-Mont/G. oxydans sensor and examination of the electrochemical mechanism were carried out in a batch system. Measurements concerning analytical characteristics, operational stability, repeatability and substrate specificity depending on the carbon source in the culture were investigated in a flow injection analysis (FIA) system. Linear ranges were found between 0.15 and 5.0 mM for the batch mode and 0.1 and 5.0 mM for the FIA system, respectively. Finally, real samples were analyzed and were compared with the results of a spectrophotometric method as reference.en_US
dc.identifier.doi10.1039/c3ra40335a
dc.identifier.endpage7519en_US
dc.identifier.issn2046-2069
dc.identifier.issn2046-2069en_US
dc.identifier.issue20en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage7513en_US
dc.identifier.urihttps://doi.org/10.1039/c3ra40335a
dc.identifier.urihttps://hdl.handle.net/11454/47064
dc.identifier.volume3en_US
dc.identifier.wosWOS:000318302900050en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherRoyal Soc Chemistryen_US
dc.relation.ispartofRsc Advancesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.titleAmine intercalated clay surfaces for microbial cell immobilization and biosensing applicationsen_US
dc.typeArticleen_US

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