OPTIMIZATION OF THERMOSTABLE ALPHA-AMYLASE PRODUCTION FROM GEOBACILLUS SP. D413

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dc.contributor.authorCaliskan Ozdemir, Sennur
dc.contributor.authorColeri Cihan, Arzu
dc.contributor.authorKilic, Tugba
dc.contributor.authorCokmus, Cumhur
dc.date.accessioned2019-10-27T23:09:07Z
dc.date.available2019-10-27T23:09:07Z
dc.date.issued2016
dc.departmentEge Üniversitesien_US
dc.description.abstractThe qualitative and quantitative a-amylase production capacities of six thermophilic bacilli were screened. Geobacillus sp. D413 was selected for enzyme optimization, as it displayed higher alpha-amylase activity. The maximum enzyme activities of D413 and G. stearothermophilus ATCC 12980(T) were observed at the time of 72 h. While the optimal pH of medium for bacterial growth and enzyme production of D413 (pH 7.0) differed from ATCC 12980(T) (pH 8.0), the optimal temperature for enzyme production was 55 degrees C for both. The effects of various carbon and nitrogen sources were determined by changing their concentrations. The highest bacterial growth and enzyme production were sustained by the starch and maltose containing medium. Both bacterial growth and enzyme production were inhibited by NH4Cl. D413 and ATCC 12980T amylases showed optimal activity at 65 degrees C, pH 9.0 and at 65 degrees C, pH 7.5, respectively. They remained active over temperature and pH ranges of 45-75 degrees C and 4.0-10.5. Their activities retained 65% and 54% when incubated at 75 degrees C for 10 min and 98-86.5% and 95-84.5% at pH 4.0-10.5 for 15 h at 37 degrees C. In conclusion, the alpha-amylase production conditions of D413 have been optimized which can be useful in biotechnological processes such as hydrolysis of starch to glucose.en_US
dc.identifier.doi10.15414/jmbfs.2016.6.1.689-694
dc.identifier.endpage694en_US
dc.identifier.issn1338-5178
dc.identifier.issn1338-5178en_US
dc.identifier.issue1en_US
dc.identifier.startpage689en_US
dc.identifier.urihttps://doi.org/10.15414/jmbfs.2016.6.1.689-694
dc.identifier.urihttps://hdl.handle.net/11454/52525
dc.identifier.volume6en_US
dc.identifier.wosWOS:000458341700012en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherSlovak Univ Agriculture Nitraen_US
dc.relation.ispartofJournal of Microbiology Biotechnology and Food Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectThermostableen_US
dc.subjectalpha-Amylaseen_US
dc.subjectProductionen_US
dc.subjectOptimizationen_US
dc.subjectGeobacillus sp.en_US
dc.titleOPTIMIZATION OF THERMOSTABLE ALPHA-AMYLASE PRODUCTION FROM GEOBACILLUS SP. D413en_US
dc.typeArticleen_US

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