Ionic Liquid Modified Single-use Electrode Developed for Voltammetric Detection of miRNA-34a and its Application to Real Samples

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dc.contributor.authorYaralı, E.
dc.contributor.authorKanat, E.
dc.contributor.authorErac, Y.
dc.contributor.authorErdem, A.
dc.date.accessioned2020-12-01T11:52:45Z
dc.date.available2020-12-01T11:52:45Z
dc.date.issued2020
dc.departmentEge Üniversitesien_US
dc.description.abstractThe ionic liquid (IL) modified chemically activated (CA) pencil graphite electrodes (PGEs) were developed for label-free voltammetric detection of miRNA-34a, and implemented to the real samples. Firstly, the electrochemical characterization of unmodified PGE, CA-PGE, IL-PGE and IL-CA-PGE was performed by cyclic voltammetry (CV) as well as their DNA binding capacity was studied by electrochemical impedance spectroscopy (EIS) technique. The microscopic characterization of the surface of each electrodes was investigated by scanning electron microscopy (SEM). Differential pulse voltammetry (DPV) technique was used for measuring the oxidation signal of guanine in order to perform a label-free voltammetric monitoring of a full-match hybridization specific to miRNA-34a. The selectivity of biosensor was tested against to miRNA-155, miRNA-660 as well as to the mismatch sequence of miRNA-34a. The further selectivity of this proposed biosensor was studied in the mixture of samples containing miRNA-34a with other miRNAs (1 : 1). The voltammetric detection of miRNA-34a was also explored in the artificial serum medium as fetal bovine serum (FBS) and also in total RNA samples isolated from HUH-7 human hepatocellular carcinoma cell line. © 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheimen_US
dc.description.sponsorshipTürkiye Bilimler Akademisien_US
dc.description.sponsorshipA.E. would like to express her gratitude to the Turkish Academy of Sciences (TUBA) as a Principal member for its partial support.en_US
dc.identifier.doi10.1002/elan.201900353
dc.identifier.endpage393en_US
dc.identifier.issn1040-0397
dc.identifier.issn1040-0397en_US
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-85073926769en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage384en_US
dc.identifier.urihttps://doi.org/10.1002/elan.201900353
dc.identifier.urihttps://hdl.handle.net/11454/61634
dc.identifier.volume32en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherWiley-VCH Verlagen_US
dc.relation.ispartofElectroanalysisen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectIonic liquidsen_US
dc.subjectmiRNAen_US
dc.subjectnucleic acid hybridizationen_US
dc.subjectpencil graphite electrodeen_US
dc.subjectvoltammetryen_US
dc.titleIonic Liquid Modified Single-use Electrode Developed for Voltammetric Detection of miRNA-34a and its Application to Real Samplesen_US
dc.typeArticleen_US

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