Label-Free Electrochemical Detection of MicroRNA-122 in Real Samples by Graphene Modified Disposable Electrodes

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dc.contributor.authorKilic, Tugba
dc.contributor.authorKaplan, Merve
dc.contributor.authorDemiroglu, Sibel
dc.contributor.authorErdem, Arzum
dc.contributor.authorOzsoz, Mehmet
dc.date.accessioned2019-10-27T22:58:16Z
dc.date.available2019-10-27T22:58:16Z
dc.date.issued2016
dc.departmentEge Üniversitesien_US
dc.description.abstractIn the present work, an electrochemical nucleic acid biosensor has been designed for the purpose of detection of miR-122 in real samples, i.e cell lysates. The fabrication of the biosensor has been done first by immobilization of complemantary anti-miR-122 to the surfaces of the graphene (GRP) modified pencil graphite (PGEs) electrodes then solid phase hybridization with either synthetic miR-122 or miR-122 included in total RNA isolated from HUH-7 cell line. The characterization of GRP modification onto the PGE surface has been proved via Raman spectroscopy analysis, Electrochemical Impedance Spectroscopy (EIS) and Differential Pulse Voltammetry (DPV). The intrinsic guanine oxidation signal measured via DPV and the charge transfer resistance, R-ct values recorded via electrochemical circle fit option of EIS have been used for hybridization detection. The proposed biosensor with the limit of detection (LOD) 1 pmol is applicable for analysis of certain miRNAs as well as miR-122 from total RNAs isolated from cell lysates. (C) 2016 The Electrochemical Society. All rights reserved.en_US
dc.identifier.doi10.1149/2.0481606jes
dc.identifier.endpageB233en_US
dc.identifier.issn0013-4651
dc.identifier.issn1945-7111
dc.identifier.issn0013-4651en_US
dc.identifier.issn1945-7111en_US
dc.identifier.issue6en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpageB227en_US
dc.identifier.urihttps://doi.org/10.1149/2.0481606jes
dc.identifier.urihttps://hdl.handle.net/11454/51496
dc.identifier.volume163en_US
dc.identifier.wosWOS:000373985300050en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElectrochemical Soc Incen_US
dc.relation.ispartofJournal of the Electrochemical Societyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.titleLabel-Free Electrochemical Detection of MicroRNA-122 in Real Samples by Graphene Modified Disposable Electrodesen_US
dc.typeArticleen_US

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