Long-term storage of microalgae: determination of optimum cryopreservation conditions

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dc.authoridDemirel, Zeliha/0000-0003-3675-7315
dc.authoriddeniz, irem/0000-0002-1171-8259
dc.authoridConk Dalay, Meltem/0000-0002-1718-7292
dc.authorscopusid56174254800
dc.authorscopusid16202502500
dc.authorscopusid16203196800
dc.authorscopusid6504178688
dc.authorwosidImamoglu, Esra/ABB-5532-2020
dc.authorwosidDemirel, Zeliha/AAG-4733-2019
dc.contributor.authorDeniz, Irem
dc.contributor.authorDemirel, Zeliha
dc.contributor.authorImamoglu, Esra
dc.contributor.authorConk-Dalay, Meltem
dc.date.accessioned2023-01-12T19:58:31Z
dc.date.available2023-01-12T19:58:31Z
dc.date.issued2022
dc.departmentN/A/Departmenten_US
dc.description.abstractMaintenance of eukaryotic microalgae strains for the long term is generally carried out using serial subculture techniques which require labour, time and cost. Cryopreservation techniques provide long-term storage of up to years for numerous microorganism strains and cell cultures. Ssu930ijn vbvbhnn8;l,n is related to a successfully designed mass and heat transfer balance throughout the cell. In this study, optimization of the cryopreservation process was carried out for two commercially used microalgal strains. The parameters to be optimized were DMSO percentage (0-25%), incubation time (1-15 min) and cryopreservation term (7-180 days) using a central composite design (CCD). Long-term storage up to 123.17 and 111.44 days corresponding to high cell viabilities was achieved for Chlorella vulgaris and Neochloris texensis, respectively. Generated models were found to be in good agreement with experimental results. The study also revealed holistic results for storage of microalgal strains in a stable state for industrial applications.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [113Z202]en_US
dc.description.sponsorshipThis work was supported by the Scientific and Technological Research Council of Turkey (TUBITAK), Grant number 113Z202.en_US
dc.identifier.doi10.1017/S0025315422000479
dc.identifier.endpage284en_US
dc.identifier.issn0025-3154
dc.identifier.issn1469-7769
dc.identifier.issn0025-3154en_US
dc.identifier.issn1469-7769en_US
dc.identifier.issue3-4en_US
dc.identifier.scopus2-s2.0-85141956639en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage276en_US
dc.identifier.urihttps://doi.org/10.1017/S0025315422000479
dc.identifier.urihttps://hdl.handle.net/11454/76936
dc.identifier.volume102en_US
dc.identifier.wosWOS:000834644500001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherCambridge Univ Pressen_US
dc.relation.ispartofJournal Of The Marine Biological Association Of The United Kingdomen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectChlorella vulgarisen_US
dc.subjectcryopreservationen_US
dc.subjectmicroalgaeen_US
dc.subjectNeochloris texensisen_US
dc.subjectoptimizationen_US
dc.subjectLipid-Accumulationen_US
dc.subjectMarine Microalgaeen_US
dc.subjectViabilityen_US
dc.subjectProteomeen_US
dc.subjectIsolateen_US
dc.subjectStepsen_US
dc.titleLong-term storage of microalgae: determination of optimum cryopreservation conditionsen_US
dc.typeArticleen_US

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