Neuroprotective Effects of Different Lavandula stoechas L. Extracts Against Hydrogen Peroxide Toxicity in vitro
Küçük Resim Yok
Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Asian Network Scientific Information-Ansinet
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Background and Objective: Cell damage caused by oxidative stress is one of the mechanisms that has been implicated for a long time in various neurodegenerative disorders such as Parkinson's and Alzheimer's diseases. Lavandula stoechas has been used in European-Iranian traditional medicine for the treatment of various diseases, primarily cardiovascular and neurodegenerative disorders. In this study, the neuroprotective effects of Lavandula stoechas L extracts against H2O2 toxicity in SH-SY5Y cells were investigated in vitro. Materials and Methods: Several extracts were prepared from the flowering branches of the L. stoechas. The neuroprotective activity of the extracts against H2O2 damage in SH-SY5Y cells and their effects on apoptotic processes were evaluated based on cell viability against H2O2 toxicity, colony formation capacity and effect on apoptotic protein levels. Results: It was determined that pretreatment with 1-10 ppm doses of L. stoechas extracts (2-24 hrs) had a significant protective effect against 250 mu M H2O2 toxicity. It was shown for the first time that L. stoechas extracts exhibited a significant protective effect for colony formation at 1-10 ppm doses and protected the colony numbers by 50% against H2O2 toxicity. In addition, it was determined that they were able to exert anti-apoptotic effects by decreasing Bax levels and increasing Bcl-2, thereby decreasing the Bax/Bcl-2 protein ratio. Conclusion: In this study, neuroprotective activities of different L. stoechas extracts were compared for the first time. Total methanol, ethyl acetate and water (infusion) extracts have shown potent neuroprotective effects and the cellular processes underlying its effects have been proven by these preliminary data.
Açıklama
Anahtar Kelimeler
Lavandula stoechas, neuroprotection, SH-SY5Y cells, oxidative stress, viability, colony formation, apoptosis, Induced Oxidative Stress, Medicinal-Plants, Essential Oils, Apoptosis, Damage, Cells, Variability, Neurons, Flowers
Kaynak
International Journal Of Pharmacology
WoS Q Değeri
Q4
Scopus Q Değeri
Cilt
18
Sayı
5
