Expression profiling of stem cell signaling alters with spheroid formation in CD133(high)/CD44(high) prostate cancer stem cells

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dc.contributor.authorOktem, Gulperi
dc.contributor.authorBilir, Ayhan
dc.contributor.authorUslu, Ruchan
dc.contributor.authorInan, Sevinc V.
dc.contributor.authorDemiray, Sirin B.
dc.contributor.authorAtmaca, Harika
dc.contributor.authorAyla, Sule
dc.contributor.authorSercan, Ogun
dc.contributor.authorUysal, Aysegul
dc.date.accessioned2019-10-27T22:14:53Z
dc.date.available2019-10-27T22:14:53Z
dc.date.issued2014
dc.departmentEge Üniversitesien_US
dc.description.abstractCancer stem cells (CSC) isolated from multiple tumor types differentiate in vivo and in vitro when cultured in serum; however, the factors responsible for their differentiation have not yet been identified. The first aim of the present study was to identify CD133(high)/CD44(high) DU145 prostate CSCs and compare their profiles with non-CSCs as bulk counterparts of the population. Subsequently, the two populations continued to be three-dimensional multicellular spheroids. Differentiation was then investigated with stem cell-related genomic characteristics. Polymerase chain reaction array analyses of cell cycle regulation, embryonic and mesenchymal cell lineage-related markers, and telomerase reverse transcriptase (TERT) and Notch signaling were performed. Immunohistochemistry of CD117, Notch1, Jagged1, Delta1, Sox2, c-Myc, Oct4, KLF4, CD90 and SSEA1 were determined in CSC and non-CSC monolayer and spheroid subcultures. Significant gene alterations were observed in the CD133(high)/CD44(high) population when cultured as a monolayer and continued as spheroid. In this group, marked gene upregulation was determined in collagen type 9 a1, Islet1 and cyclin D2. Jagged1, Delta-like 3 and Notch1 were respectively upregulated genes in the Notch signaling pathway. According to immunoreactivity, the staining density of Jagged1, Sox2, Oct4 and Klf-4 increased significantly in CSC spheroids. Isolated CSCs alter their cellular characterization over the course of time and exhibit a differentiation profile while maintaining their former surface antigens at a level of transcription or translation. The current study suggested that this differentiation process may be a mechanism responsible for the malignant process and tumor growth.en_US
dc.description.sponsorshipEge University Scientific Research Project FundEge University [Ege-BAP 2008 Tip-019]en_US
dc.description.sponsorshipThe authors would like to thank Professor Benjamin Bonavida for his editorial expertise; Assistant Professor Ogun Sercan for statistical analysis; Ummu Guven and Turker Cavusoglu for their support in Cell Culture Laboratory maintenance and inter-laboratory correlations; and Tayyibe Yeni and Merve Kursuner for flow cytometry and cell-sorting experiments in AREL. The current study was funded by a grant from the Ege University Scientific Research Project Fund (no. Ege-BAP 2008 Tip-019).en_US
dc.identifier.doi10.3892/ol.2014.1992
dc.identifier.endpage2109en_US
dc.identifier.issn1792-1074
dc.identifier.issn1792-1082
dc.identifier.issn1792-1074en_US
dc.identifier.issn1792-1082en_US
dc.identifier.issue6en_US
dc.identifier.startpage2103en_US
dc.identifier.urihttps://doi.org/10.3892/ol.2014.1992
dc.identifier.urihttps://hdl.handle.net/11454/50087
dc.identifier.volume7en_US
dc.identifier.wosWOS:000336555200071en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherSpandidos Publ Ltden_US
dc.relation.ispartofOncology Lettersen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectcancer stem cellen_US
dc.subjectstem cell-related genesen_US
dc.subjectspheroiden_US
dc.subjectprostate canceren_US
dc.titleExpression profiling of stem cell signaling alters with spheroid formation in CD133(high)/CD44(high) prostate cancer stem cellsen_US
dc.typeArticleen_US

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