Activation-based catalase enzyme electrode and its usage for glucose determination

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dc.contributor.authorAkbayirli, Pinar
dc.contributor.authorAkyilmaz, Erol
dc.date.accessioned2019-10-27T19:37:00Z
dc.date.available2019-10-27T19:37:00Z
dc.date.issued2007
dc.departmentEge Üniversitesien_US
dc.description.abstractIn this study, a novel type amperometric biosensor, which is based on the activation of catalase enzyme by glucose, was developed and used for the sensitive determination of glucose. For the preparation of the biosensor catalase enzyme was immobilized in gelatin by using cross-linking agent glutaraldehyde and fixed on a pre-treated teflon membrane of a dissolved oxygen probe. Glucose was used as an activator for the catalase enzyme and determination of glucose is based on the assay of the differences on the catalase activity of the biosensor on the oxygenmeter in the absence and the presence of glucose in the reaction medium. The responses of the activation based catalase biosensor have a linear relation to glucose concentrations and good measurement correlation between 0.5 and 5.0 mu M with 2 min response time. In the optimization studies of the biosensor the most suitable catalase amount were found as 1324 U cm(-2) and also phosphate buffer (pH: 7.0; 50 mM) and 35 degrees C were obtained as the optimum working conditions. For the characterization studies of the biosensor some parameters such as activator and interference effects of some substances on the biosensor response, reproducibility and operational stability were performed.en_US
dc.identifier.doi10.1080/00032710701689073
dc.identifier.endpage3372en_US
dc.identifier.issn0003-2719
dc.identifier.issn0003-2719en_US
dc.identifier.issue16-18en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage3360en_US
dc.identifier.urihttps://doi.org/10.1080/00032710701689073
dc.identifier.urihttps://hdl.handle.net/11454/39923
dc.identifier.volume40en_US
dc.identifier.wosWOS:000251880900030en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Incen_US
dc.relation.ispartofAnalytical Lettersen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectcatalaseen_US
dc.subjectenzyme electrodeen_US
dc.subjectactivation based biosensoren_US
dc.subjectglucoseen_US
dc.subjectbiosensoren_US
dc.titleActivation-based catalase enzyme electrode and its usage for glucose determinationen_US
dc.typeArticleen_US

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