Determination of alpha-2,6 sialic acid in developmental stages of Galleria mellonella (Lepidoptera)

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dc.contributor.authorSaribek, Buget
dc.contributor.authorErden, Secil
dc.contributor.authorKaracali, Sabire
dc.date.accessioned2019-10-27T20:50:43Z
dc.date.available2019-10-27T20:50:43Z
dc.date.issued2009
dc.departmentEge Üniversitesien_US
dc.description.abstractSialic acids are the terminal glycan structures of many glycoproteins and glycolipids, and they play a key role in many biological processes. Sialic acids can be classified according to their linkages to underlying glycans as alpha-2,3-, alpha-2,6-, and alpha-2,8 sialic acids. In this study, the expression level and distribution of alpha-2,6 linked sialic acid in the third and final larval, mature prepupa, and pupa stages of Galleria mellonella were investigated by SNA (Sambucus nigra agglutinin) lectin immunofluorescence and lectin blot analysis. The immunofluorescence analysis showed that levels of alpha-2,6 linked sialic acid were high in larval stages, especially the last larval stage, lower in mature prepupa and absent in pupa. The results of lectin blotting with SNA were consistent with immunofluorescence results. From these results, it could be inferred that the level of alpha-2,6 linked terminal sialic acid in insect tissues declines during development and aging.en_US
dc.identifier.doi10.1080/07924259.2009.9652288
dc.identifier.endpage52en_US
dc.identifier.issn0792-4259
dc.identifier.issn0792-4259en_US
dc.identifier.issue1en_US
dc.identifier.startpage45en_US
dc.identifier.urihttps://doi.org/10.1080/07924259.2009.9652288
dc.identifier.urihttps://hdl.handle.net/11454/43031
dc.identifier.volume53en_US
dc.identifier.wosWOS:000269224700005en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherInt Science Services/Balaban Publishersen_US
dc.relation.ispartofInvertebrate Reproduction & Developmenten_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGlycoproteinen_US
dc.subjectsialic aciden_US
dc.subjectlectinen_US
dc.subjectSNAen_US
dc.subjectinsecten_US
dc.titleDetermination of alpha-2,6 sialic acid in developmental stages of Galleria mellonella (Lepidoptera)en_US
dc.typeArticleen_US

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