An Electrochemical Biosensor Platform for Testing of Dehydroepiandrosterone 3-Sulfate (DHEA-S) as a Model for Doping Materials

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dc.contributor.authorBalaban, Simge
dc.contributor.authorDurmus, Ceren
dc.contributor.authorAydindogan, Eda
dc.contributor.authorGumus, Zinar Pinar
dc.contributor.authorTimur, Suna
dc.date.accessioned2019-10-27T09:41:49Z
dc.date.available2019-10-27T09:41:49Z
dc.date.issued2019
dc.departmentEge Üniversitesien_US
dc.description.abstractEndogenous steroids such as dehydroepiandrosterone (DHEA) and dehydroepiandrosterone 3-sulfate (DHEA-S) have commonly used as doping materials by athletes and to date novel techniques are needed for detection of these molecules. In this study, antibody-based electrochemical biosensor has developed for testing level of the DHEA-S. For this aim, gold surfaces were initially modified with cysteamine (Cys) and then, DHEA-S antibody was immobilized on the surface via glutaraldehyde (GA) as a crosslinking agent. The stepwise modification of electrode surface was monitored by using various electrochemical techniques such as cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Linear range was determined as 2.5-100 ng/mL DHEA-S using differential pulse voltammetry (DPV) technique, as well. Moreover, repeatability (+/- S.D.), coefficient of variation (%) and limit of detection (LOD) values were calculated as 0.033, 1.030 and 3.971, respectively. Also, DHEA-S in synthetic serum and urine samples were successfully determined with standard addition method and confirmation analysis were performed with liquid chromatography quadrupole-time of flight mass spectrometry (LC-QTOF/MS) system. The selectivity was studied with the addition of some interfering molecules (testosterone, bovine serum albumin (BSA), cholesterol, uric acid, lactic acid, codein (COD), ascorbic acid, DHEA). Consequently, this work is proposed as practical, innovative and cost-effective technique that can be easily adapted for the miniaturized form for the analysis of other doping substances as well as DHEA-S for the future works.en_US
dc.description.sponsorshipEge University Scientific Research Project Coordination (BAP)Ege University [18-EGE-MATAL-001]; [16-DPT-001]en_US
dc.description.sponsorshipThis study was supported by Ege University Scientific Research Project Coordination (BAP) coded 18-EGE-MATAL-001. All experiments were conducted in Ege University Central Research Test and Analysis Laboratory Application and Research Center (EGE-MATAL). Also, throughout all experiments, facilities provided by 16-DPT-001 project were used.en_US
dc.identifier.doi10.1002/elan.201900413
dc.identifier.issn1040-0397
dc.identifier.issn1521-4109
dc.identifier.issn1040-0397en_US
dc.identifier.issn1521-4109en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1002/elan.201900413
dc.identifier.urihttps://hdl.handle.net/11454/28686
dc.identifier.wosWOS:000483184500001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherWiley-V C H Verlag Gmbhen_US
dc.relation.ispartofElectroanalysisen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAffinity-based sensoren_US
dc.subjectElectrochemical biosensoren_US
dc.subjectDopingen_US
dc.subjectDehydroepiandrosterone (DHEA)en_US
dc.subjectDehydroepiandrosterone 3-sulfate (DHEA-S)en_US
dc.titleAn Electrochemical Biosensor Platform for Testing of Dehydroepiandrosterone 3-Sulfate (DHEA-S) as a Model for Doping Materialsen_US
dc.typeArticleen_US

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