Catechol-Attached Polypeptide with Functional Groups as Electrochemical Sensing Platform for Synthetic Cannabinoids

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dc.contributor.authorDurmus, Ceren
dc.contributor.authorAydindogan, Eda
dc.contributor.authorGumus, Zinar Pinar
dc.contributor.authorEndo, Takeshi
dc.contributor.authorYamada, Shuhei
dc.contributor.authorCoskunol, Hakan
dc.contributor.authorYagci, Yusuf
dc.date.accessioned2020-12-01T12:02:44Z
dc.date.available2020-12-01T12:02:44Z
dc.date.issued2020
dc.departmentEge Üniversitesien_US
dc.description.abstractHerein, we first constructed a functional surface using a catechol-attached polypeptide (CtP) for the detection of JWH-018 (N-4-hydroxypentyl metabolite). K2 antibody was then incorporated to the polymer via covalent cross-linker. Step-by-step modifications on the glassy carbon electrode surface were characterized by electrochemical measurements such as differential pulse voltammetry, cyclic voltammetry, impedance spectroscopy, and X-ray photoelectron spectroscopy. Linearity and the limit of detection for JWH-018 (N-4-hydroxypentyl metabolite) were determined as 10-500 ng/mL with an equation of y = 0.0018x + 0.136 (R-2 = 0.993) and 5.892 ng/mL, respectively. the selectivity of the biosensor was evaluated with different interfering molecules (methamphetamine, codeine, and cocaine). Finally, the biosensor was successfully used in the determination of JWH-018 (N-4-hydroxypentyl metabolite) in spiked synthetic urine samples, and a high-performance liquid chromatography (HPLC) system was used as a reference method to confirm the sample application. the results show that this biosensor platform can be applied to detect other JWH series of synthetic cannabinoids with high sensitivity and accuracy.en_US
dc.description.sponsorshipTurkish Scientific and Research CouncilTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [117Z152]; Istanbul Technical University Research Fund; Republic of Turkey, Ministry of DevelopmentTurkiye Cumhuriyeti Kalkinma Bakanligi [2016K121190, 2010K120810]en_US
dc.description.sponsorshipThe authors would like to thank the Turkish Scientific and Research Council (Project No. 117Z152) and Istanbul Technical University Research Fund for financial support. the Republic of Turkey, Ministry of Development, is also acknowledged (Project Grant Nos. 2016K121190 and 2010K120810) for providing the infrastructure to Ege University Central Research, Testing and Analysis Laboratory, Research and Application Center (EGE-MATAL).en_US
dc.identifier.doi10.1021/acsapm.9b00730
dc.identifier.endpage177en_US
dc.identifier.issn2637-6105
dc.identifier.issn2637-6105en_US
dc.identifier.issue2en_US
dc.identifier.startpage172en_US
dc.identifier.urihttps://doi.org/10.1021/acsapm.9b00730
dc.identifier.urihttps://hdl.handle.net/11454/62745
dc.identifier.volume2en_US
dc.identifier.wosWOS:000514258700007en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherAmer Chemical Socen_US
dc.relation.ispartofAcs Applied Polymer Materialsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectsurface modificationen_US
dc.subjectbiosensoren_US
dc.subjectabused drug detectionen_US
dc.subjectJWH-018 (N-4-hydroxypentyl metabolite)en_US
dc.subjectsynthetic cannabinoidsen_US
dc.titleCatechol-Attached Polypeptide with Functional Groups as Electrochemical Sensing Platform for Synthetic Cannabinoidsen_US
dc.typeArticleen_US

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