Electrochemical Detection of Interaction between Dacarbazine and Nucleic Acids in Comparison to Agarose Gel Electrophoresis

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dc.contributor.authorKanat, Erkin
dc.contributor.authorEksin, Ece
dc.contributor.authorKaracicek, Bilge
dc.contributor.authorErac, Yasemin
dc.contributor.authorErdem, Arzum
dc.date.accessioned2019-10-27T10:04:11Z
dc.date.available2019-10-27T10:04:11Z
dc.date.issued2018
dc.departmentEge Üniversitesien_US
dc.description.abstractAnticancer drug Dacarbazine (DCB) has been used for the treatment for metastatic malignant melanoma. In our study, the biomolecular interaction between DCB and DNA was investigated electrochemically for the first time using single-walled carbon nanotube (SWCNT) modified disposable pencil graphite electrode (PGEs). The SWCNT modified PGEs were optimized for advanced DNA sensing based on the response related to changes at the guanine oxidation signal. The oxidation signals of DCB and guanine were respectively measured before/after surface confined interaction process using differantial pulse voltammetry (DPV) in the same voltammetric scale. The experimental parameters, such as DCB concentration and interaction time of DCB with fsDNA were studied. The detection of interaction between DCB and nucleic acids was also studied via electrochemical impedance spectroscopy (EIS) and gel electrophoresis. In addition, the effect of time-dependent manner from 1min to 10min on the interaction of DCB with nucleic acids was explored upon to the changes at guanine signal of fsDNA as well as PCR samples by DPV technique.en_US
dc.description.sponsorshipTurkish Academy of Sciences (TUBA)Turkish Academy of Sciencesen_US
dc.description.sponsorshipA.E. would like to express her gratitude to the Turkish Academy of Sciences (TUBA) as a Principal member for its partial support.en_US
dc.identifier.doi10.1002/elan.201800064
dc.identifier.endpage1574en_US
dc.identifier.issn1040-0397
dc.identifier.issn1521-4109
dc.identifier.issn1040-0397en_US
dc.identifier.issn1521-4109en_US
dc.identifier.issue7en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1566en_US
dc.identifier.urihttps://doi.org/10.1002/elan.201800064
dc.identifier.urihttps://hdl.handle.net/11454/30211
dc.identifier.volume30en_US
dc.identifier.wosWOS:000438025200043en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherWiley-V C H Verlag Gmbhen_US
dc.relation.ispartofElectroanalysisen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectDacarbazineen_US
dc.subjectDrug-DNA interactionen_US
dc.subjectElectrochemical Biosensoren_US
dc.subjectGel Electrophoresisen_US
dc.subjectPencil graphite electrodeen_US
dc.subjectSingle-Walled Carbon Nanotubesen_US
dc.titleElectrochemical Detection of Interaction between Dacarbazine and Nucleic Acids in Comparison to Agarose Gel Electrophoresisen_US
dc.typeArticleen_US

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