Inhibitory effects of propolis on human osteogenic sarcoma cell proliferation mediated by caspase patway [Kaspaz yolagi{dotless} ile yönlendirilen i·nsan osteojenik sarcoma hücre çogalmasi{dotless}na propolisin baski{dotless}layi{dotless}ci{dotless} etkisi]

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dc.contributor.authorOzdal Kurt F.
dc.contributor.authorVatansever H.S.
dc.contributor.authorSorkun K.
dc.contributor.authorDeliloglu Gurhan S.I.
dc.contributor.authorTurkoz E.
dc.contributor.authorGencay O.
dc.contributor.authorSalih B.
dc.date.accessioned2019-10-26T22:35:49Z
dc.date.available2019-10-26T22:35:49Z
dc.date.issued2010
dc.departmentEge Üniversitesien_US
dc.description.abstractA natural product Propolis, is a resinous material gathered by honeybees from the buds and bark of certain trees and plants. Propolis contains various chemical components of biological activities, including antimutagenic, antioxidant, antibacterial, antiviral and anticarsinogenic. Therefore, the aim of this study is to investigate the antiapoptotic effect of propolis extracts (PE) using caspase pathway in the human osteogenic sarcoma cell line SAOS-2 in culture. The extracts which produced in ecologic environment were taken from the Hacettepe University, Beytepe Campus area-Ankara were used. Seven different PE at 0.5, 0.25, 0.125 and 0.063 mg/ml were added to SAOS-2 cell line for two days incubation. For cell proliferation and cytotoxicty analyses MTT, for apoptotic cell death determination TUNEL method, for distribution of caspase 6, caspase 8 and caspase 9 indirect immunocytochemistry analyses were used. After MTT analyses, the most effect was observed PE 7 at the 0.125 mg/ml dilution. The number of TUNEL positive cells was more detectable at PE 4 and 5 at the 0.063 mg/ml, and PE 7 at the 0.125 mg/ml dilutions. The immunoreactivity of caspase 6 was stronger than caspase 8 and 9. Moreover, density of caspase 6 staining was much better especially in PE 7 at the 0.125 mg/ml dilution. In conclusion, the mechanisms of apoptosis induction by PE may appear via caspase pathway because of its anticanserogenic effect. PE may be usefull in the cancer treatment protocol.en_US
dc.identifier.endpage404en_US
dc.identifier.issn1300-6045
dc.identifier.issn1300-6045en_US
dc.identifier.issue3en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage397en_US
dc.identifier.urihttps://hdl.handle.net/11454/19854
dc.identifier.volume16en_US
dc.indekslendigikaynakScopusen_US
dc.language.isotren_US
dc.relation.ispartofKafkas Universitesi Veteriner Fakultesi Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectApoptosisen_US
dc.subjectCaspaseen_US
dc.subjectCell lineen_US
dc.subjectMTTen_US
dc.subjectPropolisen_US
dc.subjectSAOS-2en_US
dc.titleInhibitory effects of propolis on human osteogenic sarcoma cell proliferation mediated by caspase patway [Kaspaz yolagi{dotless} ile yönlendirilen i·nsan osteojenik sarcoma hücre çogalmasi{dotless}na propolisin baski{dotless}layi{dotless}ci{dotless} etkisi]en_US
dc.typeArticleen_US

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