Detection of Leishmania RNA virus 2 in Leishmania species from Turkey

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dc.contributor.authorNalcaci, Muhammed
dc.contributor.authorKarakus, Mehmet
dc.contributor.authorYilmaz, Bahtiyar
dc.contributor.authorDemir, Samiye
dc.contributor.authorOzbilgin, Ahmet
dc.contributor.authorOzbel, Yusuf
dc.contributor.authorToz, Seray
dc.date.accessioned2019-10-27T09:42:56Z
dc.date.available2019-10-27T09:42:56Z
dc.date.issued2019
dc.departmentEge Üniversitesien_US
dc.description.abstractBackground: Leishmania RNA virus (LRV) is a double-stranded RNA (dsRNA) virus infecting some Leishmania strains and triggering a destructive hyperinflammatory response in mammalian hosts in the New World. There is limited knowledge of the presence of this virus in Old World Leishmania species and its role in the outcome of the disease. We aimed to investigate the presence of LRV in Leishmania species/strains from Turkey. Methods: Twenty-nine previously identified Leishmania isolates (24 L. tropica, 2 L. infantum, 3 L. major) were examined for LRV positivity using dsRNA visualization in agarose gel after total nucleic acid extraction and RQ-deoxyribonuclease treatment and amplification of a 526 bp fragment of the LRV2-specific RNA-dependent RNA polymerase gene by reverse transcription polymerase chain reaction. Results: Ten (7 L. tropica [24.13%], 3 L. major [10.34%]) of the 29 Leishmania strains gave positive results for LRV. Basic Local Alignment Search Tool analysis showed that all these viruses are LRV2-1. LRV2 was detected for the first time in L. tropica strains in the present study. Conclusions: The clinical manifestation and resistance status of the disease can be different depending on the host and parasite species/strains. The presence of LRV2 may be one of the factors contributing the course of disease. Further studies are needed to elucidate the specific role of LRV2, as it may be a potential target for effective treatment strategies.en_US
dc.description.sponsorshipEge University Scientific Research Projects Coordination UnitEge University [17-FEN-045]; TUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [114S999]en_US
dc.description.sponsorshipThis work was supported by the Ege University Scientific Research Projects Coordination Unit (17-FEN-045) and partially by TUBITAK Project No. 114S999.en_US
dc.identifier.doi10.1093/trstmh/trz023
dc.identifier.endpage417en_US
dc.identifier.issn0035-9203
dc.identifier.issn1878-3503
dc.identifier.issn0035-9203en_US
dc.identifier.issn1878-3503en_US
dc.identifier.issue7en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage410en_US
dc.identifier.urihttps://doi.org/10.1093/trstmh/trz023
dc.identifier.urihttps://hdl.handle.net/11454/28824
dc.identifier.volume113en_US
dc.identifier.wosWOS:000482524300006en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherOxford Univ Pressen_US
dc.relation.ispartofTransactions of the Royal Society of Tropical Medicine and Hygieneen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectLeishmaniaen_US
dc.subjectLeishmania majoren_US
dc.subjectLeishmania RNA virusen_US
dc.subjectLeishmania tropicaen_US
dc.subjectLRV2en_US
dc.titleDetection of Leishmania RNA virus 2 in Leishmania species from Turkeyen_US
dc.typeArticleen_US

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