Active Phytochemical Detecting, Antioxidant, Cytotoxic, Apoptotic Activities of Ethyl Acetate and Methanol Extracts of Galium aparine L.

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dc.contributor.authorAslanturk, Ozlem Sultan
dc.contributor.authorCelik, Tulay Askin
dc.contributor.authorKarabey, Burcin
dc.contributor.authorKarabey, Fatih
dc.date.accessioned2019-10-27T11:21:57Z
dc.date.available2019-10-27T11:21:57Z
dc.date.issued2017
dc.departmentEge Üniversitesien_US
dc.description.abstractThe aim of this study was to detect active phytochemicals in ethyl acetate (EAE) and methanol (ME) extracts of Galium aparine L. and determine in vitro antioxidant, cytotoxic and apoptotic activities of these extracts. The phytochemicals present in the plant were determined using standard methods and HPLC. EAE and ME of Galium aparine arial parts were tested for antioxidant (by using DPPH radical scavenging, H2O2 scavenging and metal chelating assays), cytotoxic (by using MTT assay on MCF-7 breast cancer and Caco-2 colon cancer cells), and apoptotic activity (DNA diffusion assay on MCF-7 breast cancer and Caco-2 colon cancer cells). Human peripheral blood lymphocytes were used as control cells. As a result of phytochemical screening of EAE and ME of Galium aparine arial parts, phenols, tannins, alkaloids, anthraquinones and saponins were detected. Furthermore, rutin, a flavonoid phytochemical was found in both of the extracts by HPLC analysis. EAE and ME have cytotoxic and apoptotic activity on human MCF-7 breast cancer and Caco-2 colon cancer cells. These activities may depend on polarity of solvents used in the extraction and on concentration of the extracts. EAE and ME showed strong DPPH center dot radical scavenging, very low H2O2 scavenging and metal chelation activity. Besides, EAE showed higher cytotoxic and apoptotic effect on human peripheral lymphocytes compared to ME. The G. aparine EAE and ME led cytotoxicity and apoptosis in human MCF-7 breast cancer and Caco-2 colon cancer cells.en_US
dc.description.sponsorshipAdnan Menderes University Scientific Research FoundationAdnan Menderes University [FEF-13001]en_US
dc.description.sponsorshipWe acknowledge to Adnan Menderes University Scientific Research Foundation for financial support (Project No: FEF-13001). Thanks to Dr. Ozkan EREN for botanical identification of Galium aparine L.en_US
dc.identifier.doi10.9734/BJPR/2017/32762
dc.identifier.issn2231-2919
dc.identifier.issn2231-2919en_US
dc.identifier.issue6en_US
dc.identifier.urihttps://doi.org/10.9734/BJPR/2017/32762
dc.identifier.urihttps://hdl.handle.net/11454/32978
dc.identifier.volume15en_US
dc.identifier.wosWOS:000400481000002en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherSciencedomain Inten_US
dc.relation.ispartofBritish Journal of Pharmaceutical Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectApoptosisen_US
dc.subjectCaco-2 cellsen_US
dc.subjectcytotoxicityen_US
dc.subjectDPPH radical scavenging activityen_US
dc.subjectG. aparine L.en_US
dc.subjectMCF-7 cellen_US
dc.subjectphytochemical screeningen_US
dc.titleActive Phytochemical Detecting, Antioxidant, Cytotoxic, Apoptotic Activities of Ethyl Acetate and Methanol Extracts of Galium aparine L.en_US
dc.typeArticleen_US

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