Anticancer activities of bioactive peptides derived from rice husk both in free and encapsulated form in chitosan

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dc.authoridCELIKTAS, Melih Soner Soner/0000-0003-0597-5133
dc.authoridBudak, Gozde/0000-0001-5796-7202
dc.authoridIlhan Ayisigi, Esra/0000-0003-1880-4261
dc.authorscopusid57200659470
dc.authorscopusid57230069500
dc.authorscopusid25722917300
dc.authorscopusid36091705900
dc.authorscopusid22837401200
dc.authorwosidCELIKTAS, Melih Soner Soner/E-4538-2010
dc.authorwosidYesil-Celiktas, Ozlem/AAI-5713-2020
dc.authorwosidBudak, Gozde/AAR-6804-2020
dc.contributor.authorIlhan-Ayisigi, Esra
dc.contributor.authorBudak, Gozde
dc.contributor.authorCeliktas, Melih Soner
dc.contributor.authorSevimli-Gur, Canan
dc.contributor.authorYesil-Celiktas, Ozlem
dc.date.accessioned2023-01-12T19:55:22Z
dc.date.available2023-01-12T19:55:22Z
dc.date.issued2021
dc.departmentN/A/Departmenten_US
dc.description.abstractCereal grain-derived protein hydrolysates exert beneficial effects for human health, thus utilization of by-products with high protein content has drawn attention. In this study, hot water extraction of rice husk has been optimized to obtain protein hydrolysate with the highest anticancer activity. The optimum protein value was obtained as 2.40 g/L corresponding to 43.2 g protein/kg dried rice husk under the conditions of 60 degrees C, 2.0 mL/min flow rate and pH 10.0. The protein hydrolysate was encapsulated with chitosan, where the mean particle size of protein hydrolysate (0.3 %) loaded chitosan nanoparticles was 256.4 +/- 33. 4 nm with 89 % encapsulation efficiency and a 65% release at the end of 6 days. Cytotoxicity assays showed that the lowest cell viabilities have been achieved with A549 and MCF7 cells with IC50 values of 1.98 and 3.58 mu g/mL, respectively, whereas nuclear fragmentation and apoptotic bodies were observed through Hoechst 33342 staining. The cytotoxic effect might be associated with the wide variety of peptide/protein subunits ranging from 10 kDa to more than 180 kDa in the protein hydrolysate of rice husk. (C) 2021 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipKocaeli University [KOU-BAP-2016-081, KOU-BAP-2018-149]en_US
dc.description.sponsorshipThe research funds provided by Kocaeli University (KOU-BAP-2016-081 and KOU-BAP-2018-149) are highly appreciated. Special thanks to Mr. Ahmet Yilmaz who provided the rice husk from Ozserhat Gida Sanayi Tic. Ltd. Sti, Edirne, Turkey. We are grateful to Dilan Yerli for helping with SDS-PAGE, Prof. Dr. Kemal Sami Korkmaz for providing access to his lab at Ege University and Pelin Saglam-Metiner for her contributions to fluorescent staining assays.en_US
dc.identifier.doi10.1016/j.jiec.2021.08.006
dc.identifier.endpage391en_US
dc.identifier.issn1226-086X
dc.identifier.issn1876-794X
dc.identifier.issn1226-086Xen_US
dc.identifier.issn1876-794Xen_US
dc.identifier.scopus2-s2.0-85113379875en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage381en_US
dc.identifier.urihttps://doi.org/10.1016/j.jiec.2021.08.006
dc.identifier.urihttps://hdl.handle.net/11454/76667
dc.identifier.volume103en_US
dc.identifier.wosWOS:000693222000019en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevier Science Incen_US
dc.relation.ispartofJournal Of Industrial And Engineering Chemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPeptideen_US
dc.subjectRice husken_US
dc.subjectHot water extractionen_US
dc.subjectCytotoxicityen_US
dc.subjectChitosan nanoparticleen_US
dc.subjectWater Extractionen_US
dc.subjectProtein Profileen_US
dc.subjectNanoparticlesen_US
dc.subjectCanceren_US
dc.subjectAntioxidanten_US
dc.subjectSilicaen_US
dc.subjectBranen_US
dc.titleAnticancer activities of bioactive peptides derived from rice husk both in free and encapsulated form in chitosanen_US
dc.typeArticleen_US

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