A preliminary study to develop a lateral flow assay using recombinant GRA1 protein for the diagnosis of toxoplasmosis in stray cats

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dc.authorscopusid57201674137
dc.authorscopusid23003351400
dc.authorscopusid57219922088
dc.authorscopusid57330182000
dc.authorscopusid57221117593
dc.authorscopusid56495590200
dc.authorscopusid57407199500
dc.contributor.authorDeğirmenci, Döşkaya, A.
dc.contributor.authorCan, H.
dc.contributor.authorGül, A.
dc.contributor.authorKarakavuk, T.
dc.contributor.authorGüvendi, M.
dc.contributor.authorKarakavuk, M.
dc.contributor.authorGül, C.
dc.date.accessioned2024-08-25T18:32:26Z
dc.date.available2024-08-25T18:32:26Z
dc.date.issued2023
dc.departmentEge Üniversitesien_US
dc.description.abstractToxoplasma gondii is a protozoan parasite that may infect many mammals including humans. Cats are one of the main sources of infection for humans. Therefore, routine screening of cats with tests that are inexpensive, rapid, and do not require sophisticated laboratory equipment is important. In this study, a lateral flow assay (LFA) was designed to rapidly diagnose toxoplasmosis in cats. For this purpose, we selected GRA1 protein of T. gondii due to its high antigenicity in diagnostic and vaccine studies. We further analyzed the immunological properties of GRA1 protein using in silico tools. Then, we expressed and purified recombinant GRA1 (rGRA1) protein and used it during the development of LFA to detect toxoplasmosis in serum samples (n = 40) of cats. According to the results, rGRA1 protein has negative GRAVY value, high aliphatic index, alpha helix, random coil and 12 B cell epitopes. The in silico data supported the high antigenic properties of rGRA1 protein and showed that it can be a good antigen candidate for LFA. Among 30 cat positive serum samples, 27 were found positive by the LFA while seronegative sera (n = 10) were negative by the LFA. The preliminary data showed that the LFA has high sensitivity (90 %) and specificity (100 %). When we used high responsive cat sera (i.e. sera that have optical density > 0.5 with ELISA) the sensitivity value reached 100 %. These results showed that rGRA1 protein is a good candidate to develop a LFA for rapid diagnosis of toxoplasmosis in cats. © 2023 Elsevier Ltden_US
dc.description.sponsorshipEge Üniversitesi: TGA-2021-22468en_US
dc.description.sponsorshipThis study was supported by a project given by the Ege University Scientific Research Projects Coordination Unit (Project no.: TGA-2021-22468 ) to A.D.D.en_US
dc.identifier.doi10.1016/j.cimid.2023.102057
dc.identifier.issn0147-9571
dc.identifier.scopus2-s2.0-85169004179en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.cimid.2023.102057
dc.identifier.urihttps://hdl.handle.net/11454/100239
dc.identifier.volume101en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevier Ltden_US
dc.relation.ispartofComparative Immunology, Microbiology and Infectious Diseasesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmz20240825_Gen_US
dc.subjectCaten_US
dc.subjectLateral flow assayen_US
dc.subjectRecombinant GRA1en_US
dc.subjectToxoplasma gondiien_US
dc.titleA preliminary study to develop a lateral flow assay using recombinant GRA1 protein for the diagnosis of toxoplasmosis in stray catsen_US
dc.typeArticleen_US

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