High-Yield Biocatalysis of Baicalein 7-O-beta-d-Glucuronide to Baicalein Using Soluble Helix pomatia-Derived beta-Glucuronidase in a Chemically Defined Acidic Medium

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dc.contributor.authorMuderrisoglu, Cahit
dc.contributor.authorYesil-Celiktas, Ozlem
dc.date.accessioned2019-10-27T09:44:15Z
dc.date.available2019-10-27T09:44:15Z
dc.date.issued2019
dc.departmentEge Üniversitesien_US
dc.description.abstractBaicalein, showing stronger pharmacological activity, can be obtained by removal of the distal glucuronic acid (GluA) from baicalein 7-O--D-glucuronide (baicalin). In the present study, a chemically defined reaction medium comprised of mildly acidic (pH 4.5, 37 degrees C) aqueous solution, was formulated for biotransformation of baicalin to baicalein using acidic Helix pomatia derived beta-glucuronidase (HP-GUS), an untested biocatalyst source. The biotransformation was carried out as a batchwise process within an optimised reaction cocktail (with 5% dimethylformamide, v/v) by a 4-h HP-GUS (250 unit/ml) incubation of baicalin (60ppm) and resulted in a promising conversion ratio of 99% without any by-product formation. The formulated reaction system may offer a novel and efficient alternative for bioproduction of baicalein, which can be vital for pharmaceutical applications. [GRAPHICS] .en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [113M050]en_US
dc.description.sponsorshipThe financial support provided by the Scientific and Technological Research Council of Turkey (TUBITAK, 113M050) is highly appreciated. Special thanks to Dr Ismail Hakki Akgun from Ege University Bioengineering Department for his guidanceas toUPLC analysis of the reaction samples.en_US
dc.identifier.doi10.1007/s10562-019-02745-3
dc.identifier.endpage1709en_US
dc.identifier.issn1011-372X
dc.identifier.issn1572-879X
dc.identifier.issn1011-372Xen_US
dc.identifier.issn1572-879Xen_US
dc.identifier.issue6en_US
dc.identifier.startpage1701en_US
dc.identifier.urihttps://doi.org/10.1007/s10562-019-02745-3
dc.identifier.urihttps://hdl.handle.net/11454/28971
dc.identifier.volume149en_US
dc.identifier.wosWOS:000465575500021en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofCatalysis Lettersen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBiotransformationen_US
dc.subjectScutellaria baicalensis Georgien_US
dc.subjectBaicalinen_US
dc.subjectBaicaleinen_US
dc.subjectbeta-Glucuronidaseen_US
dc.titleHigh-Yield Biocatalysis of Baicalein 7-O-beta-d-Glucuronide to Baicalein Using Soluble Helix pomatia-Derived beta-Glucuronidase in a Chemically Defined Acidic Mediumen_US
dc.typeArticleen_US

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