Diatom cultivation and lipid productivity for non-cryopreserved and cryopreserved cells

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dc.contributor.authorDemirel Z.
dc.contributor.authorDemirkaya C.
dc.contributor.authorImamoglu E.
dc.contributor.authorConk Dalay M.
dc.date.accessioned2019-10-26T21:19:17Z
dc.date.available2019-10-26T21:19:17Z
dc.date.issued2016
dc.departmentEge Üniversitesien_US
dc.description.abstractMany freshwater and marine algae can be cryopreserved, but typically with lower postthaw viability levels. However, most of the algae groups (dinoflagellates, cryptophytes, synurophytes, and raphidophytes) cannot be successfully cryopreserved in these days. Marine diatoms can be cryopreserved and frequently have shown great viability. The aim of this study is to compare the cultivation and lipid productivity for non-cryopreserved and cryopreserved marine diatom cells. Diatoms preserved in the EGEMACC (Ege University Microalgae Culture Collection) are usually maintained by serial sub-culturing. In this study, the cryopreservation of marine diatom algae (Amphora cf. capitellata, Cylindrotheca closterium, Nanofrustulum shiloi) using the passive freezing system procedure was studied. Investigation into the cause of the freezing injury at the cellular level was made at different salt concentrations. Passive freezing method used in sea salts liquid media at the percentage of 1%, 2% and 3% containing cryoprotectant of 10% Me2SO for six months in liquid nitrogen. C. closterium was obtained with the highest viability however N. shiloi was revival extended period of time. All of the diatom cells were grown in 1 L sterile bottle containing 900 mL of F/2 medium under the light intensity of 20 µmol photons m-2 s-1 at 22 ± 2 °C with the air flow rate of 1 L min-1 for 15 days. The growth rate and biomass productivity were determined at the end of the batch production process. Also, lipid content of A. capitellata was obtained at the highest concentration compared to that of the other diatoms. © 2016, Eesti Pollumajandusulikool. All rights reserved.en_US
dc.identifier.endpage1273en_US
dc.identifier.issn1406894X
dc.identifier.issn1406-894Xen_US
dc.identifier.issue4en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1266en_US
dc.identifier.urihttps://hdl.handle.net/11454/16623
dc.identifier.volume14en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherEesti Pollumajandusulikoolen_US
dc.relation.ispartofAgronomy Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAmphora cf. capitellataen_US
dc.subjectCryopreservationen_US
dc.subjectCylindrotheca closteriumen_US
dc.subjectGrowth rateen_US
dc.subjectLipid contenten_US
dc.subjectNanofrustulum shiloien_US
dc.titleDiatom cultivation and lipid productivity for non-cryopreserved and cryopreserved cellsen_US
dc.typeArticleen_US

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