Commercially suitable pectin methylesterase from Valencia orange peels [Valencia portakal kabuklari{dotless}ndan ticari olabilecek pektin metilesteraz eldesi]

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dc.contributor.authorŞimşek S.
dc.contributor.authorYemenicioglu A.
dc.date.accessioned2019-10-26T22:45:19Z
dc.date.available2019-10-26T22:45:19Z
dc.date.issued2010
dc.departmentEge Üniversitesien_US
dc.description.abstractA simple and effective procedure was developed to extract pectin methylesterase (PME) from Valencia orange peels. Orange peels contain 25-34 µmol of COOH min-1 g-1 of peel PME activity. The enzyme was ionically bound to cell walls and could not be extracted with water. This enables removal of water soluble pectic substances and oils from peels via homogenization and washing with water before enzyme extraction. Enzyme extraction can be conducted simply by addition of suitable amounts of NaCl (optimum: 10 g of NaCl 100 g-1 of extraction mixture) to peel homogenate and stirring (optimum: 30 min at 200 rpm). The PME extracted from orange peels contains almost the same amount of heat-stable and heat-labile fraction, and the enzymes cannot be activated by mild heating. A slight activation of enzyme (almost 20%) was achieved by adding 1 mM CaCl2 to enzyme extracts, but this agent was inhibitory at higher concentrations. The extracts stabilized by Na-benzoate and K-sorbate maintained more than 90% of their PME activity at 4 °C for at least 5 months. The obtained PME was successfully used to prepare low-methoxyl citrus pectin used in edible film formation in the presence of CaCl2. This study shows the potential of using Valencia orange peels as a source of commercial PME. © TÜBİTAK.en_US
dc.identifier.doi10.3906/tar-0903-7
dc.identifier.endpage119en_US
dc.identifier.issn1300011X
dc.identifier.issn1300-011Xen_US
dc.identifier.issue2en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage109en_US
dc.identifier.urihttps://doi.org/10.3906/tar-0903-7
dc.identifier.urihttps://hdl.handle.net/11454/20084
dc.identifier.volume34en_US
dc.indekslendigikaynakScopusen_US
dc.language.isotren_US
dc.publisherTurkiye Klinikleri Journal of Medical Sciencesen_US
dc.relation.ispartofTurkish Journal of Agriculture and Forestryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectExtractionen_US
dc.subjectOrange peelen_US
dc.subjectPectin methylesteraseen_US
dc.subjectStabilityen_US
dc.subjectValencia orangeen_US
dc.titleCommercially suitable pectin methylesterase from Valencia orange peels [Valencia portakal kabuklari{dotless}ndan ticari olabilecek pektin metilesteraz eldesi]en_US
dc.typeArticleen_US

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