Laccase: production by Trametes versicolor and application to denim washing

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dc.contributor.authorPazarlioglu, NK
dc.contributor.authorSariisik, M
dc.contributor.authorTelefoncu, A
dc.date.accessioned2019-10-27T18:58:36Z
dc.date.available2019-10-27T18:58:36Z
dc.date.issued2005
dc.departmentEge Üniversitesien_US
dc.description.abstractLaccase activity was monitored in synthetic media supplemented with various phenolics. Phenol was the most effective inducer. The white rot basidiomycete Trametes versicolor grown in non-induced and phenol induced conditions, was tested for production of laccase (TvLac), lignin peroxidase (LiP), manganese peroxidase (MnP), arylalcohol oxidase (AAO) and polyphenol oxidase (PPO). Although no PPO, LiP and AAO were observed in the culture filtrates, TvLac and MnP activities were detected at the fourth and fifth day of incubation, respectively. In phenol induced cultures, the maximum enzyme activity was enhanced about 20-fold with regard to control cultures. Laccase production at optimum phenol concentration was investigated by monitoring enzyme activity, protein content, glucose consumption and biomass and it was determined that laccase production is non-growth associated. The optimum pH of the enzyme was 4.5. Kinetic constants of TvLac was determined as K. value 0.6 1mM and a V-max of 8264 U/L (R-2 = 0.99). Laccase, isolated from T.versicolor, was also used for denim washing without a mediator. TvLac was more effective than commercial laccase with a mediator. (c) 2004 Elsevier Ltd. All rights reserved.en_US
dc.identifier.doi10.1016/j.procbio.2004.06.052
dc.identifier.endpage1678en_US
dc.identifier.issn1359-5113
dc.identifier.issn1873-3298
dc.identifier.issn1359-5113en_US
dc.identifier.issn1873-3298en_US
dc.identifier.issue5en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1673en_US
dc.identifier.urihttps://doi.org/10.1016/j.procbio.2004.06.052
dc.identifier.urihttps://hdl.handle.net/11454/37508
dc.identifier.volume40en_US
dc.identifier.wosWOS:000227569000026en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevier Sci Ltden_US
dc.relation.ispartofProcess Biochemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectTrametes versicoloren_US
dc.subjectlaccaseen_US
dc.subjectphenol inductionen_US
dc.subjectbiobleachingen_US
dc.subjectdenim washingen_US
dc.titleLaccase: production by Trametes versicolor and application to denim washingen_US
dc.typeArticleen_US

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