Oligomeric Thiosemicarbazones as Novel Immobilization Matrix in Biosensing Applications

Basit Öğe Kaydı
dc.contributor.authorSeven, Basak
dc.contributor.authorDemirdoven, Turker
dc.contributor.authorYildirim, Hatice
dc.contributor.authorDemirkol, Dilek Odaci
dc.contributor.authorSubasi, Elif
dc.contributor.authorSahin, Elif
dc.contributor.authorTimur, Suna
dc.date.accessioned2019-10-27T21:43:21Z
dc.date.available2019-10-27T21:43:21Z
dc.date.issued2013
dc.departmentEge Üniversitesien_US
dc.description.abstractThiosemicarbazone (TSC) matrices are constructed by electrochemically oligomerization on the graphite electrodes to investigate their matrix properties in terms of biomolecule immobilization for the biosensor applications. Amino functionalized oligomeric TSCs were successfully applied for the glucose oxidase (GOx) immobilization using glutaraldehyde (GA) as the crosslinker. Scanning electron microscopy (SEM) and fourier transform infrared spectroscopy (FT-IR) measurements are used to characterize the modified surface. Electrochemical measurements of the GOx biosensor were performed at ambient conditions at 0.7vs. Ag/AgCl electrode by following the oxygen consumption resulting from the enzyme activity, in the presence of glucose substrate. Linearity, operational stabilities and repeatability are investigated in detail, as well as optimization of pH and enzyme amount,. The optimized biosensor shows a very good linearity between 0.05mM and 1.0mM glucose with a 10s response time. Kinetic parameters such as K-m and I-max are also calculated. Besides the batch configuration, the analytical characteristics of the system were also evaluated in flow injection analysis (FIA) mode. Finally, the new biosensor configuration was applied for glucose determination on a real sample.en_US
dc.description.sponsorshipDokuz Eylul University, the Scientific Research CouncilDokuz Eylul University [2012.KB.FEN.053]en_US
dc.description.sponsorshipThe authors would like to thank Dokuz Eylul University, the Scientific Research Council (Project No: 2012.KB.FEN.053) for financial support.en_US
dc.identifier.doi10.1080/10601325.2013.768148
dc.identifier.endpage398en_US
dc.identifier.issn1060-1325
dc.identifier.issn1520-5738
dc.identifier.issn1060-1325en_US
dc.identifier.issn1520-5738en_US
dc.identifier.issue4en_US
dc.identifier.startpage392en_US
dc.identifier.urihttps://doi.org/10.1080/10601325.2013.768148
dc.identifier.urihttps://hdl.handle.net/11454/47071
dc.identifier.volume50en_US
dc.identifier.wosWOS:000315657000005en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Incen_US
dc.relation.ispartofJournal of Macromolecular Science Part A-Pure and Applied Chemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectEnzyme immobilizationen_US
dc.subjectimmobilization matrixen_US
dc.subjectthiosemicarbazoneen_US
dc.titleOligomeric Thiosemicarbazones as Novel Immobilization Matrix in Biosensing Applicationsen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu